文献类型：期刊文献

中文题名：正交试验优化铁棒锤生物碱提取工艺

英文题名：Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method

作者：杜弢[1];陈红刚[1];林丽[1];魏学明[1]

第一作者：杜弢

机构：[1]甘肃中医学院,兰州730000

第一机构：甘肃中医药大学

年份：2011

卷号：17

期号：3

起止页码：20

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2008】；

基金：2009年甘肃省教育厅科研项目(0906B-07)

语种：中文

中文关键词：正交试验;铁棒锤生物碱;提取工艺;酸碱滴定法

外文关键词：orthogonal design ; total alkaloids of Aconitum flavum ; extraction technology ; acid-base titrations

摘要：目的:优选铁棒锤生物碱的最佳提取工艺。方法:选择溶剂、提取次数、洗涤次数为考察因素,以总生物碱的含量为考察指标,采用正交试验法优选铁棒锤生物碱的最佳提取工艺。结果:铁棒锤生物碱的最佳提取工艺为浸提次数3次,提取溶剂为乙醚-三氯甲烷(3∶1),洗涤1次。结论:优选得到的提取工艺稳定性好、得率高、简便可行。
Objective：To optimize the extraction technology for total alkaloids in the root of Aconitum flarum. Method： Dissolvent, times of extraction and washing were the test factors and the total alkaloids were the test index. The orthogonal design was used to optimize the extraction technology of total alkaloids. Result： The optimal extraction method was as follows ： the ether ： chloroform （3 ： 1 ） as dissolvent, extraction 3 times and washing once. Conclusion： The technology after optimization had a good stability, the yield of total alkaloids was high, and the method was easy to operate.