文献类型：期刊文献

中文题名：红芪多糖对高糖培养下雪旺细胞Bcl-2/Caspase-3信号通路的影响

英文题名：Effect of Hedysarum Polysaccharides on Bcl-2/Caspase-3 Signaling Pathway of Schwann Cells Cultured in High Glucose

作者：陈彦旭[1];金智生[1];何流[2];徐长青[1];金彩云[1];张磊[1];姜晓雪[1];郇鹏飞[3]

第一作者：陈彦旭

机构：[1]甘肃中医药大学,兰州730000;[2]贵州中医药大学第二附属医院,贵阳550000;[3]上海中医药大学,上海200000

第一机构：甘肃中医药大学

年份：2022

卷号：28

期号：6

起止页码：71

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金项目(81660777)。

语种：中文

中文关键词：红芪多糖;糖尿病周围神经病变;雪旺细胞;凋亡反应;B淋巴细胞瘤-2(Bcl-2);半胱氨酸天冬氨酸蛋白水解酶-(3 Caspase-3);Bcl-2相关X蛋白(Bax)

外文关键词：Hedysarum polysaccharides;diabetic peripheral neuropathy;Schwann cells;apoptotic response;B-cell lymphoma 2(Bcl-2);cysteinyl aspartate-specific protease 3(Caspase-3);Bcl-2-associated X protein(Bax)

摘要：目的:通过观察红芪多糖(HPS)对高糖培养下雪旺细胞(SCs)B细胞淋巴瘤-2(Bcl-2)、半胱氨酸天冬氨酸蛋白水解酶-3(Caspase-3)、Bcl-2相关X蛋白(Bax)信号通路的影响,探讨HPS在治疗糖尿病周围神经病变(DPN)过程中可能的作用机制。方法:选取出生5~7 d的SD乳鼠4只,随机分为正常组、高糖组、HPS+高糖组、α-硫辛酸(α-LA)+高糖组,从坐骨神经部位提取SCs,放入37℃,5%CO_(2)培养箱中培养,待细胞达整瓶的80%后,细胞增殖与活性检测(CCK-8)法筛适宜高糖,HPS及α-LA干预的实验浓度,蛋白免疫印迹法(Western blot),实时荧光定量聚合酶链式反应(Real-time PCR)检测Bcl-2、Bax、Caspase-3蛋白与mRNA的表达情况,膜联蛋白V-异硫氰酸荧光素(Annexin V-FITC)/碘化丙锭(PI)法流式细胞术检测各组SCs的凋亡率。结果:Western blot,Real-time PCR检测结果显示,与正常组比较,高糖组Bcl-2蛋白及mRNA表达水平降低,Bax、Caspase-3蛋白及mRNA表达水平升高,差异有统计学意义(P<0.01);与高糖组比较,HPS+高糖组、α-LA+高糖组的Bcl-2蛋白及mRNA表达水平明显升高,Bax、Caspase-3蛋白及mRNA表达水平明显降低,差异有统计学意义(P<0.01)。Annexin V/PI法流式细胞术检测结果显示,与正常组比较,高糖组SCs凋亡率明显增加;与高糖组比较,HPS+高糖组和α-LA+高糖组SCs凋亡率显著降低,差异有统计学意义(P<0.01)。结论:HPS可以减轻SCs凋亡反应状态,其作用机制可能与抑制Bcl-2/Caspase-3信号通路激活有关。
Objective:To observe the effects of Hedysarum polysaccharides(HPS)on the signaling pathways of B-cell lymphoma 2(Bcl-2),cysteinyl aspartate-specific protease 3(Caspase-3),and Bcl-2-associated X protein(Bax)in Schwann cells(SCs)cultured in high glucose,and explore the possible mechanism of HPS against diabetic peripheral neuropathy(DPN).Method:Four SD suckling mice aged5-7 days were randomly divided into a normal group,a high-glucose group,an HPS+high-glucose group,and anα-lipoic acid(α-LA)+high-glucose group.SCs were extracted from the sciatic nerve and cultured in a 37℃,5%CO2 incubator.After the cells reached 80%confluence,Cell Counting Kit-8(CCK-8)was used to screen the experimental concentrations suitable for high glucose,HPS,andα-LA interventions.Western blot and Real-time polymerase chain reaction(Real-time PCR)were used to detect the protein and mRNA expression of Bcl-2,Bax,and Caspase-3.The apoptosis rate of SCs was detected by flow cytometry using Annexin V-fluorescein isothiocyanate(FITC)/propidium iodide(PI).Result:As revealed by Western blot and real-time PCR,compared with the normal group,the high-glucose group showed reduced protein and mRNA expression of Bcl-2 and increased protein and mRNA expression of Bax and Caspase-3(P<0.01).Compared with the high-glucose group,the HPS+high-glucose group and theα-LA+high-glucose group showed increased protein and mRNA expression of Bcl-2 and decreased protein and mRNA expression of Bax and Caspase-3(P<0.01).As displayed by the results of flow cytometry using Annexin V/PI,compared with the normal group,the high-glucose group showed increased apoptosis rate;compared with the high-glucose group,the HPS+high-glucose group and theα-LA+high-glucose group showed reduced apoptosis rate(P<0.01).Conclusion:HPS can alleviate the apoptotic response of SCs,and its mechanism may be related to the inhibition of the activation of the Bcl-2/Caspase-3 signaling pathway.