文献类型：期刊文献

英文题名：Lycium barbarum polysaccharides protect human trophoblast HTR8/SV neo cells from hydrogen peroxide-induced oxidative stress and apoptosis

作者：Li, Jing[1];Ding, Zhongjun[2];Yang, Yue[3];Mao, Baohong[1];Wang, Yanxia[1];Xu, Xiaoying[4]

第一作者：Li, Jing

通信作者：Xu, XY[1]

机构：[1]Gansu Prov Matern & Child Care Hosp, Dept Women & Childrens Med Ctr, Lanzhou 730050, Gansu, Peoples R China;[2]Gansu Prov Matern & Child Care Hosp, Reprod Med Ctr, Lanzhou 730050, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Discipline Physiol, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Prov Matern & Child Care Hosp, Perinatal Ctr, 143 North St, Lanzhou 730050, Gansu, Peoples R China

第一机构：Gansu Prov Matern & Child Care Hosp, Dept Women & Childrens Med Ctr, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Prov Matern & Child Care Hosp, Perinatal Ctr, 143 North St, Lanzhou 730050, Gansu, Peoples R China.

年份：2018

卷号：18

期号：3

起止页码：2581

外文期刊名：MOLECULAR MEDICINE REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000442890400007)】；

语种：英文

外文关键词：Lycium barbarum polysaccharides; human trophoblast cells; HTR8/SVneo cells; hydrogen peroxide; oxidative stress; cell apoptosis

摘要：Pregnancy complications are associated with abnormal cytotrophoblast differentiation and invasion. Hydrogen peroxide (H2O2) is an important mediator of oxidative ischemia/reperfusion stress in the placenta. Lycium barbarum polysaccharides (LBP) have been demonstrated to counteract oxidative free radicals. The effects of LBP in trophoblast HTR8/SVneo cells injured with H2O2 were examined. A cell counting kit-8 assay was performed to detect the effect of LBP at different concentrations on the proliferative ability of H2O2 injured trophoblast cells. Flow cytometry was used to determine the levels of reactive oxygen species (ROS), mitochondria membrane potential (MMP) disruption and apoptosis. Superoxide dismutase (SOD) activity and lactate dehydrogenase (LDH) leakage into the supernatant was detected by ultraviolet spectrophotometry. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were performed to detect the expression of apoptosis-associated factors, including survivin, hypoxia inducible factor 1-alpha (HIF1-alpha), Bcl-2 apoptosis regulator (Bcl-2), Bcl-2 associated X apoptosis regulator (Bax). The results revealed that LBP protected the proliferative ability of trophoblast cells injured with H2O2 in a dose-dependent manner. LBP inhibited the oxidative stress induced by H2O2, by reducing ROS and LDH levels and increasing SOD activity. Additionally, LBP decreased MMP disruption and cell apoptosis induced by H2O2, by increasing the mRNA and protein expression of survivin, HIF1-alpha and Bcl-2 and decreasing Bax expression. Therefore, it was concluded that LBP protected human trophoblast cells from H2O2-induced oxidative stress and cell apoptosis via regulation of apoptosis-associated factor expression. It will provide a novel strategy for the treatment of pregnancy complications.