文献类型：期刊文献

中文题名：当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的影响

英文题名：Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82

作者：张艳霞[1];蔺兴遥[1];安方玉[1];张正顺[2];陈绍仪[3];周佳玮[1];张延英[1]

第一作者：张艳霞

机构：[1]甘肃中医药大学/甘肃省中药质量与标准研究重点实验室培育基地;[2]甘肃省中医院白银分院;[3]白银市第二人民医院

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：23

期号：1

起止页码：29

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：白银市科技计划项目(2013-2-76Y);甘肃中医学院中青年科研基金项目(2305016601)

语种：中文

中文关键词：当归挥发油;人肺腺癌GLC-82细胞;细胞凋亡

外文关键词：volatile oil of the angelica; human lung adenocarcinoma cell GLC-82; apoptosis

摘要：目的:探讨当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的作用。方法:MTT比色法检测当归挥发油对GLC-82细胞的细胞毒作用;流式细胞术检测GLC-82细胞凋亡;吖啶橙/溴化乙锭(AO/BE)双荧光染色法观察GLC-82细胞凋亡。结果:当归挥发油在浓度6.25~200μg/m L范围内对GLC-82细胞的增殖具有浓度依赖性抑制作用,对GLC-82细胞的IC_(50)为113.03μg/m L。流式细胞术检测结果表明,当归挥发油可诱导GLC-82细胞凋亡、坏死,且细胞凋亡、坏死率随药物浓度增高而升高。AO/BE双荧光染色法结果显示,用药组随着药物浓度的升高,细胞结构固缩加重,凋亡、坏死细胞增多。结论:当归挥发油具有诱导GLC-82细胞凋亡的作用。
AIM： To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell GLC-82. METHODS： MTT colorimetric assay was used to investigate the cytotoxic effect of human lung adenocarcinoma cell GLC-82. The cell apoptosis was analyzed by flow cytometry. AO/BE fluorescent staining was applied to observe the cell apoptosis. RESULTS： The GLC-82 cell was inhibited by volatile oil of the angelica in a concentration-dependent manner; the IC50 was 113. 03 μg/m L. The result of flow cytometry indicated volatile oil of the angelica could induce apoptosis and necrosis of GLC-82 cell,and the rates of apoptosis and necrosis were increased by the increase of drug concentration. The results of AO/BE fluorescent staining method indicated the rate of apoptosis and necrosis increased in drug group along with the increase of drug concentration. CONCLUSION： Volatile oil can induce the apoptosis of GLC-82 cell.