文献类型：期刊文献

英文题名：Tumor Inhibition Effects and Mechanisms of Angelica sinensis and Sophorae flavescentis ait Decoction Combined with Cisplatin in Xenograft Mice

作者：Yan, De-Qi[1];Liu, Yong-Qi[1];Li, Ying-Dong[1];Li, Dou[1];Cheng, Xiao-Li[1];Wu, Zhi-Wei[1]

第一作者：Yan, De-Qi

通信作者：Liu, YQ[1]

机构：[1]Gansu Tradit Chinese Med Coll, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Tradit Chinese Med Coll, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2014

卷号：15

期号：11

起止页码：4609

外文期刊名：ASIAN PACIFIC JOURNAL OF CANCER PREVENTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000338634900038)】；

基金：This study was financially supported by the national science and technology support program (NO: 2011BAI05B02), the basic scientific research fund of Gansu Province universities (NO: Gan Finance 2009-192-3) and by Youth league committee innovation fund project of Gansu college of traditional Chinese medicine. We thank the individuals and their families for their participation in this reasearch program. We are also indebted to YAN Deqi for the whole experiment and independant writing, to Doctor LIU Yongqi and Doctor LI Yingdong for financing and other support, to LI Dou for involving in animal and serum experiment, to CHENG Xiaoli for pathological and immunohistochemistry experiment and analysis, to Doctor Wu Zhiwei for RT-PCR experiment.

语种：英文

外文关键词：Angelica sinensis; Sophorae flavescentis ait; decoction; H22 cells; tumor xenografts; DDP

摘要：Background: To investigate tumor inhibition effects and mechanisms of Angelica sinensis and Sophorae flavescentis ait decoction (ASSF) combined with diamine-dichloroplatinum (DDP). Materials and Methods: Bodyweight, tumor inhibition rate and q value were calculated for single ASSF or ASSF combined with DDP on H22 carcinoma xenograft KM mice. Biochemical methods for serum LDH, AST, ALT, and AKP, ELISA method for serum HIF-1a, pathological assessemnt of thymus, immunohistochemistry detection of tumor tissue caspase3 and mutant p53 protein, and qRT-PCR detection of bax/bcl-2 mRNA were applied. Results: Compared with DDP control group, the bodyweight increased in ASSF-DDP group (p<0.01). Tumor inhibition rates for DDP, ASSF, ASSF-DDP were 62.7%. 43.7% and 71.0% respectively, with a q value of 0.90. Compared with other groups, thymus of DDP control group had obvious pathological injury (p<0.01), serum LDH, AST, ALT, AKP increased significantly in DDP control group (p<0.01), while serum HIF-1a was increased in the model control group. Compared with this latter, the expression of mutant p53 protein and bcl-2 mRNA were decreased in all treatment groups (p<0.01), but there were no statistical difference between DDP control p and ASSF-DDP groups. The expression of caspase3 protein and bax mRNA was increased in all treatment groups, with statistical differences between the DDP and ASSF-DDP groups (p<0.01). Conclusions: ASSF can inhibit bodyweight decrease caused by DDP, can inhibit tumor growth synergistically with DDP mainly through increasing serum HIF-1 alpha and pro-apoptotic molecules such as caspase 3 and bax, rather than through decreasing anti-apoptotic mutant p53 and bcl-2. ASSF can reduce DDP toxicity due to decreasing the release of LDH, AST, ALT, AKP into blood and enhancing thymus protection.