文献类型：期刊文献

中文题名：当归及当归多糖对辐射所致大鼠肝肾损伤的防护作用

英文题名：Protective effects of Angelica sinensis and Angelica sinensis polysaccharides against liver and kidney injury induced by irradiation in rats

作者：卢志伟[1];许小敏[1];王磊[1];张利英[1];何进鹏[2];丁楠[2];李程豪[1];苏韫[1];刘永琦[1,3]

第一作者：卢志伟

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃省空间辐射生物学重点实验室,中国科学院近代物理研究所,兰州730000;[3]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2017

卷号：42

期号：9

起止页码：815

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(81473457);甘肃省高等学校科研项目(2015A-095);甘肃中医药大学研究生创新基金(CX2016-16)~~

语种：中文

中文关键词：当归;当归多糖;辐射;肝损伤;肾损伤

外文关键词：Angelica sinensis; Angelica sinensis polysaccharide; radiation; liver injury; kidney injury

摘要：目的探讨当归及当归多糖对X线辐射所致SD大鼠肝、肾损伤的保护作用。方法 32只SD大鼠随机分为对照组、模型组、当归水煎液组和当归多糖组,每组8只。常规饲养14d后,灌胃给药,每日1次,连续7d。从第8天起对除对照组外其余各组大鼠进行全身X线照射,连续照射2d,总吸收剂量为6Gy,剂量率为92.26c Gy/min,辐射后第3天股动脉采血处死大鼠。采用比色分析法检测肝、肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性以及丙二醛(MDA)、过氧化脂(LPO)的含量;行HE染色并在显微镜下观察肝、肾组织病理变化;Western blotting检测大鼠肝、肾组织NF-E2相关因子2(Nrf2)蛋白的表达。结果与对照组比较,模型组大鼠肝、肾质量明显增加,组织SOD和GSH-PX活性明显降低,MDA、LPO含量明显增加,肝、肾细胞明显水肿,肝、肾组织Nrf2含量增加。与模型组比较,当归水煎液及当归多糖组肝、肾质量降低,SOD和GSH-Px性明显增加,MDA、LPO含量明显减少,肝、肾细胞水肿明显减轻,肝、肾组织中Nrf2含量降低,而当归多糖组与当归水煎液组比较差异无统计学意义。结论当归及当归多糖对辐射所致的SD大鼠肝、肾损伤具有较好的保护作用,当归的抗辐射损伤作用可能主要是通过当归多糖实现的。
Objective To investigate the protective effects of Angelica sinensis and Angelica sinensis polysaccharide against liver and kidney injury induced by irradiation of SD rats. Methods Thirty-two SD rats were randomly divided into four groups： control group, model group, Angelica decoction group and Angelica sinensis polysaccharide group. After 14 days of routine feeding, the rats were treated by intragastric administration once a day for 7 days. From the 8th day, the rats received whole body X-ray irradiation for 2 days. The total absorbed dose was 6Gy, and the dose rate was 92.26cGy/min. The rats were killed and the blood were collected from the femoral artery 3 days after irradiation. The content of superoxide dismutase （SOD）, malondialdehyde （MDA）, glutathione peroxidase （GSH-Px） and lipid peroxide （LPO） in the liver and kidney tissues was detected by colorimetric analysis. The pathological changes of the liver and kidney were observed by HE staining under microscope. The expression of Nrf2 protein in the liver and kidney of the rats was detected by Western blotting. Results Compared with control group, the weight of the liver and kidney significantly increased, the activities of SOD and GSH-Px significantly decreased, the contents of MDA and LPO significantly increased, and the liver and kidney cells showed obvious edema, and the content of Nrf2 in the liver and kidneyincreased in the model group. Compared with model group, the weight of the liver and kidney decreased, SOD and GSH-Px activities significantly increased, the contents of MDA and LPO significantly decreased, edema of the liver and kidney significantly reduced, the expression of Nrf2 in the liver and kidney tissues decreased in Angelica decoction group and Angelica sinensis polysaccharides group, while there was no difference between Angelica sinensis polysaccharide group and Angelica decoction group. Conclusion Angelica sinensis and Angelica polysaccharides have a good protective effect against liver and kidney injury induced by radiation in SD rats, which is implemented mainly through influencing the expression of free radicals. The protective effect of Angelica against radiation injury is achieved mainly by Angelica polysaccharide.