文献类型：期刊文献

中文题名：HPLC研究甘肃黄芩水煎液指纹图谱

英文题名：Study on fingerprint atlas of S. rehderiana Diels Decoction by HPLC

作者：迟栋[1];彭晓霞[1];陈晖[1]

第一作者：迟栋

机构：[1]甘肃中医学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2009

卷号：26

期号：3

起止页码：39

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

语种：中文

中文关键词：甘肃黄芩;高效液相色谱;指纹图谱

外文关键词：S. rehderiana Diels; HPLC; fingerprint atlas

摘要：目的建立甘肃黄芩水煎液的HPLC指纹图谱。方法应用Sunfire C18色谱柱,甲醇—0.85%磷酸水溶液进行梯度洗脱,流速为1 mL/min,检测波长280 nm。结果精密度、重现性、稳定性试验中共有峰相对峰面积、相对保留时间的RSD均小于3%;不同产地甘肃黄芩相似度大于0.97。结论该方法简便、实用、可靠,可以作为评价甘肃黄芩药材质量及其制剂体外指纹图谱研究的基础。
Objective To set up HPLC fingerprint atlas of S. rehderiana Diels Decoction. Methods Chromatographic conditions were available as follows Sunfire Cls column, methanol and water including 0.85 % phosphoric acid as mobile phase, flowrate was 1 mL/min, and detecting wavelength was 280 nm. Results The relative peak areas and retention time of common peak in the experiment of precision, repeatability and stability were not reach 3 %, respectively similarity of S. rehder/ana Diels collected from different origin were over 0.97. Conclusion The method is simple, practicable and reliable as a foundation method of controlling the quality of S. rehderiana Diels and the study of fingerprint atlas in vitro.