文献类型：期刊文献

中文题名：当归配方颗粒与当归饮片质量比较研究

英文题名：Comparative Study on Quality of Angelica Formula Particles and Angelica Pieces

作者：李越峰[1];张泽国[1];徐福菊[1];金辉[1];杜丽东[1];王瑞琼[1];王继龙[1];严兴科[1]

第一作者：李越峰

机构：[1]甘肃中医学院

第一机构：甘肃中医药大学

年份：2014

卷号：21

期号：4

起止页码：70

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：教育部科学技术研究重点项目(212186);甘肃省高等学校基本科研业务费专项资金(2013-2);甘肃省自然科学基金(1010RJZA212);甘肃中医学院引进人才科研启动基金(2010-3)

语种：中文

中文关键词：当归配方颗粒;当归饮片;阿魏酸;高效液相色谱法

外文关键词：Angelica formula particles;Angelica pieces;ferulic acid;HPLC

摘要：目的：通过测定当归配方颗粒中阿魏酸含量、水分和浸出物含量,为评价及控制其质量提供可靠方法,并对当归配方颗粒及当归饮片的质量进行比较研究。方法采用高效液相色谱法。色谱柱：Kromasil C18色谱柱（4.6 mm×250 mm,5μm）；流速：1.0 mL/min；检测波长：316 nm；柱温：28℃；流动相：乙腈-0.05%磷酸水梯度洗脱系统。结果阿魏酸在0.033～0.165μg（r＝0.9998）之间有良好的线性关系,平均加样回收率为98.78%,RSD＝0.99%。当归配方颗粒在各项含量指标上符合质量要求,阿魏酸含量高于当归饮片。结论当归配方颗粒中阿魏酸含量测定方法操作简单、可靠,重复性好,可作为当归配方颗粒质量控制的有效方法。
Objective To determine the content of ferulic acid, water and extract of Angelica formula particles, provide a reliable method for evaluation and quality control, and compare the quality of Angelica formula particles and Angelica pieces. Methods HPLC method was used with Kromasil C18 column （4.6 mm×250 mm, 5μm）, flow rate of 1.0 mL/min, detection wavelength of 316 nm, column temperature at 28 ℃. The mobile phase was acetonitrile-0.05%phosphoric acid in water gradient elution system. Results Ferulic acid showed good linear relationship in the range of 0.033-0.165 μg （r=0.999 8）. The average recovery was 98.78% and RSD=0.99%. Angelica formula particles met the quality requirement in the content indicators, the content of ferulic acid was higher than Angelica pieces. Conclusion The HPLC method for determination of ferulic acid in Angelica formula particles is simple and reliable, with good reproducibility, and can be used as the quality control method of Angelica formula particles.