文献类型：期刊文献

英文题名：Enhanced glioma cell death with ZnO nanorod flowers and temozolomide combination therapy through autophagy and mitophagy pathways

作者：Li, Yuanyuan[1];Ma, Yonghua[1];Li, Jingjing[2];Lu, Yan[3];Liu, Haiying[1];Gao, Min[1];Cao, Junqin[1]

第一作者：Li, Yuanyuan

通信作者：Ma, YH[1]

机构：[1]Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[3]Northwest Normal Univ, Coll Phys & Elect Engn, Lanzhou 730070, Peoples R China

第一机构：Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Peoples R China.

年份：2024

卷号：178

外文期刊名：BIOMEDICINE & PHARMACOTHERAPY

收录：;Scopus(收录号：2-s2.0-85199148417);WOS:【SCI-EXPANDED(收录号:WOS:001278895700001)】；

基金：This work was supported by the fund of Gansu Agricultural University (GAU-XKJS-2018-073, GAU-KYQD-2017RCZX-11, GAU-QDFC-2020-11) , Lanzhou Science and Technology Plan Project (2022-2-112, 2019-1-32) , and Gansu Province Natural Sciences Fund (18JR3RA16, 21JR1RA233) .

语种：英文

外文关键词：ZnO nanorod flowers; Glioma; Temozolomide; Autophagy; Mitophagy; Apoptosis

摘要：In recent years, the application of engineered NMts has significantly contributed to various biomedical fields. ZnO NMts (ZnO NMts) are widely utilized due to their biocompatibility, unique physical and chemical properties, stability, and cost-effectiveness for large-scale production. They have emerged as potential materials for anticancer applications. This study aims to study the impact of ZnO Nanorod flowers (ZnO NRfs) and their combination with temozolomide (TMZ) on glioma cells. Normal mouse microglia (BV2) will be used as a control to assess the effects on mouse glioma cells (G422) and human glioma cells (LN229). The effects of these substances were evaluated on G422 and LN229 cells through various parameters such as IC50 value, Zn2+ + accumulation, ROS production, apoptosis, mitochondrial membrane potential (MMP) depolarization, and examination of organelles like mitochondria and lysosomes. Additionally, hypoxia-inducible factor-1 alpha (HIF-1 alpha), endothelial cell PAS domain protein 1 (EPAS1), autophagy markers (LC3), mitophagy and phagocytosis marker (BNIP3) were assessed. The results demonstrated that the combination of ZnO NRfs and TMZ could influence the expression of HIF-1 alpha, EPAS1, LC3, and BNIP3 proteins, leading to mitophagy in glioma cells. This combination treatment has the potential to effectively eliminate glioma cells by activating the mitophagy pathway, which provides a good prospect for the clinical treatment of glioma.