文献类型：期刊文献

中文题名：当归红芪超滤物对冠心病大鼠心肌纤维化及HIF-1α、OPN蛋白的影响

英文题名：Effect of Radix Angelica Sinensis and Radix Hedysari Ultrafiltration on Myocardial Fibrosis and HIF-1α、OPN Protein in Rats with Coronary Heart Disease

作者：王倩[1];龚玉栓[1];马承旭[3];李淑玲[2];王洁[2];杨开燕[2];李煦煦[2];魏惠平[2]

第一作者：王倩

机构：[1]甘肃中医药大学中西医结合学院,甘肃兰州730000;[2]甘肃中医药大学附属医院心血管中心,甘肃兰州730020;[3]兰州大学第一医院内分泌科,甘肃兰州744600

第一机构：甘肃中医药大学中西医结合学院

年份：2024

卷号：35

期号：5

起止页码：1123

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：;北大核心:【北大核心2023】；CSCD:【CSCD_E2023_2024】；

基金：甘肃省自然科学基金(21JR7RA581);甘肃省中医药管理局课题(GZKZ-2020-9)。

语种：中文

中文关键词：当归红芪超滤物;冠心病;心肌纤维化;HIF-1α;OPN

外文关键词：Radix Angelica Sinensis and Radix Hedysari Ultrafiltration;Coronary heart disease;Myocardial fibrosis;HIF-1α;OPN

摘要：目的探讨当归红芪超滤物对高脂饮食诱导的冠心病大鼠心肌纤维化及HIF-1α、OPN蛋白的干预效应。方法将36只Wistar雄性大鼠随机分为空白组、模型组、瑞舒伐他汀钙片组(0.1mg·kg^(-1)·d^(-1))、当归红芪超滤物低剂量组(0.1g·kg^(-1)·d^(-1))、当归红芪超滤物中剂量组(0.3g·kg^(-1)·d^(-1))、当归红芪超滤物高剂量组(0.5g·kg^(-1)·d^(-1))。药物干预4周后ELISA法检测大鼠血清CK-MB、cTnI的表达水平,Masson染色法观察心肌组织纤维化变化,免疫组化法检测心肌组织TNF-α、MMP-9、TGF-β1蛋白的表达,免疫荧光检测心肌组织中HIF-1α、OPN蛋白的表达量。结果与空白组相比,模型组大鼠纤维化面积及程度显著,与模型组相比,当归红芪超滤物组纤维化面积及程度减轻;与空白组相比,模型组TNF-α、MMP-9、TGF-β1蛋白表达升高,与模型组相比,当归红芪超滤物组TNF-α、MMP-9、TGF-β1蛋白表达降低;与空白组相比,模型组HIF-1α表达显著降低(P<0.01)、OPN表达显著升高(P<0.01);与模型组比较,当归红芪超滤物中、高剂量组HIF-1α表达升高(P<0.05)、OPN表达降低(P<0.05)。结论当归红芪超滤物对高脂饮食诱导的冠心病大鼠心肌纤维化及HIF-1α、OPN蛋白的表达具有改善作用。
Objective To investigate the effect of Radix Angelica Sinensis and Radix Hedysari Ultrafiltration on myocardial fibrosis and HIF-1αand OPN protein in rats with coronary heart disease induced by high fat diet.Methods will Thirty-six Wistar male rats were randomly divided into blank group,model group,Rosuvastatin calcium tablet group(0.1mg·kg^(-1)·d^(-1)),Radix Angelica Sinensis and Radix Hedysari Ultrafiltration low-dose group(0.1g·kg^(-1)·d^(-1)),Radix Angelica Sinensis and Radix Hedysari Ultrafiltration low-medium dose group(0.3g·kg^(-1)·d^(-1)),and Radix Angelica Sinensis and Radix Hedysari Ultrafiltration high-dose group(0.5g·kg^(-1)·d^(-1)).After 4 weeks of drug intervention,the expression levels of serum CK-MB and cTnI were detected by ELISA,the changes of myocardial fibrosis were observed by Masson staining,the expressions of TNF-α,MMP-9 and TGF-β1 proteins in myocardial tissue were detected by immunohistochemistry,and the expressions of HIF-1αand OPN proteins in myocardial tissue were detected by immunofluorescence.Results Compared with the blank group,the fibrosis area and degree of the model group were significant,and the fibrosis area and degree of the Radix Angelica Sinensis and Radix Hedysari Ultrafiltration group were reduced.Compared with the blank group,the protein expressions of TNF-α,MMP-9 and TGF-β1 in the model group were increased,while the protein expressions of TNF-α,MMP-9 and TGF-β1 in the Radix Angelica Sinensis and Radix Hedysari Ultrafiltration group were decreased compared with the model group.Compared with blank group,HIF-1αexpression in model group was significantly decreased(P<0.01)and OPN expression was significantly increased(P<0.01).Compared with model group,HIF-1αexpression was increased(P<0.05)and OPN expression was decreased(P<0.05)in medium and high dose groups of Radix Angelica Sinensis and Radix Hedysari Ultrafiltration.Conclusion Radix Angelica Sinensis and Radix Hedysari Ultrafiltration can improve the expression of HIF-1αand OPN protein in coronary heart disease rats induced by high fat diet.