文献类型：期刊文献

英文题名：Angelica sinensis polysaccharide ameliorates chemotherapy-induced intestinal mucositis by regulating gut microbiota-mediated innate immunity and short-chain fatty acid production in Drosophila and mice

作者：Wang, Yan[1];Zhang, Yongxuan[2];Yue, Shiqi[1];Liu, Menglu[2];Li, Kexin[1];Wu, Jinhan[2];Wang, Junlong[4];Xiu, Minghui[1];He, Jianzheng[2,3]

第一作者：王艳;王琰;王燕

通信作者：Xiu, MH[1];He, JZ[2]

机构：[1]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Res & Expt Ctr, Lanzhou 730000, Peoples R China;[3]Gansu Basic Disciplines Res Ctr Dunhuang Med, Lanzhou 730000, Peoples R China;[4]Northwest Normal Univ, Coll Life Sci, Lanzhou 730000, Peoples R China

第一机构：甘肃中医药大学公共卫生学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Res & Expt Ctr, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;

年份：2026

卷号：382

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20263821485278);Scopus(收录号：2-s2.0-105050432424);WOS:【SCI-EXPANDED(收录号:WOS:001880380200001)】；

基金：This research was funded by the Gansu Province Higher Education Industry Support Programme (No. 2026CYZC-055) , Gansu Natural Sci-ence Foundation (No. 25JRRA1177) , Key Projects of the Gansu Pro-vincial Administration of Traditional Chinese Medicine (No. GZKZ-2025-53) . Lanzhou Youth Science and Technology Talent Innovation Project (No. 2024-QN-35) .

语种：英文

外文关键词：Angelica sinensis polysaccharide; Chemotherapeutic intestinal mucositis; Innate immunity response; Short-chain fatty acid

摘要：Intestinal mucositis is one of the most debilitating side effects of chemotherapeutic agents. Angelica sinensis polysaccharide (ASP), the crucial active ingredient of Angelica sinensis, has been reported to possess anti-colitis activity. However, the efficacy of ASP against chemotherapy-induced intestinal mucositis (CIM) remain to be clarified. The aim of this study using Drosophila melanogaster and mouse models was to investigate the potential effect of ASP on intestinal mucositis and its underlying mechanism. ASP significantly alleviated overall physiological and intestinal damage caused by CPT-11 in adult flies, including increased survival rate and intestinal length, improved digestive capacity, restored intestinal acid-base balance and reduced death of intestinal epithelial cells. Near Infrared (NIR) imaging indicated that ASP was absorbed through the intestine and metabolized via the hepatic and renal systems in mice. Furthermore, ASP reduced intestinal damage and restored the intestinal barrier function in CPT-11 treated mice, including increased intestinal length, elevated levels of ZO-1 and an increased number of goblet cells. Mechanistically, ASP markedly down-regulated the over-activated innate immunity by inhibiting the Toll-IMD and TLR4/NF-kappa B/MyD88 signaling pathways in CPT-11 induced flies and mice. Besides, ASP also exerted a protective effect against structural damage to the spleen induced by CPT-11. Moreover, ASP ameliorated gut microbiota imbalances and increased the levels of short-chain fatty acids (SCFAs), particularly propionate and butyrate. Fecal microbiota transplantation (FMT) further confirmed that ASP could modulate gut microbiota and protect against intestinal mucositis in mice. Collectively, these results demonstrate that ASP effectively ameliorates CIM and has the potential to serve as a novel adjunctive therapy to CPT-11.