文献类型：期刊文献

中文题名：黄芪多糖(APS)及联合顺铂(DDP)抑制小鼠Lewis肺癌复发瘤生长的机制

英文题名：Astragalus polysaccharide combined with cisplatin inhibits growth of recurrent tumor and down-regulats the expression of CD44,CD62P and osteopontin in tumor tissues in mice bearing Lewis lung cancer

作者：刘丹[1,2,3];李杨[1];王雪林[1,2,3];王彦君[1,2,3];马寅瑞[1];陈彦文[1];明海霞[1,2,3,4]

第一作者：刘丹

机构：[1]甘肃中医药大学;[2]甘肃中医药大学中西医结合研究所;[3]甘肃省高校重大疾病分子医学与中医药防治研究重点实验室;[4]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：34

期号：12

起止页码：1105

中文期刊名：细胞与分子免疫学杂志

外文期刊名：Chinese Journal of Cellular and Molecular Immunology

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；PubMed;

基金：甘肃省高校基本科研业务项目(2014);甘肃省中医药管理局资助项目(GZK-2017-6);甘肃省高等学校科研项目(2018A-046);敦煌医学与转化教育部重点实验室开放基金项目(DHYX18-13);甘肃中医药大学研究生创新基金(CX2018-33)

语种：中文

中文关键词：黄芪多糖(APS);Lewis肺癌;顺铂(DDP);CD44;CD62P;骨桥蛋白(OPN)

外文关键词：Astragalus polysaccharide;Lewis lung cancer;DDP;CD44;CD62P;OPN

摘要：目的观察黄芪多糖(APS)及联合顺铂(DDP)对小鼠Lewis肺癌术后复发瘤病理形态及转移相关蛋白CD44、CD62P和骨桥蛋白(OPN)表达的影响。方法选取C57BL/6J小鼠10只作为供瘤组,另外80只随机分为模型组、(50、100、200)μg/mL APS处理组、6 mg/kg DDP处理组、3 mg/kg DDP联合(50、100、200)μg/m L APS处理组,每组10只。取供瘤组小鼠瘤组织制备Lewis瘤单细胞悬液接种于每只小鼠左侧后肢爪垫内侧皮下10 d后,切除爪垫瘤组织,建立肺癌术后复发转移模型;造模次日起给药,第16天脱颈处死。采用HE染色法观察术后复发瘤组织病理形态,免疫组织化学染色法检测复发瘤中CD44、CD62P和OPN的表达。结果与模型组相比,各处理组复发瘤组织坏死加重,尤以DDP联合200μg/mL APS处理组更明显,且各处理组CD44、CD62P和OPN蛋白的表达均有降低,以DDP以及DDP联合(100、200)μg/m L APS处理组最为显著。结论DDP联合APS能抑制Lewis肺癌细胞生长并降低瘤组织CD44、CD62P和OPN蛋白表达。
Objective To investigate the effects of Astragalus polysaccharide( APS) combined with cisplatin( DDP) on the pathological morphology of recurrent tumor and the expression of metastasis-related proteins CD44, CD62 P and osteopontin( OPN) following Lewis lung carcinoma( LLC) surgery. Methods LLC cel suspension was injected subcutaneously into palmula of left hind limb of C57 BL/6 J mice as a tumor-supply group. The other 80 mice were randomized into 8 groups:model group,APS-treated groups at different concentrations of 50,100 and 200 μg/m L,6 mg/kg DDP-treated group,and3 mg/kg DDP combined with 50,100,200 μg/m L APS-treated groups. The palmula tumor cells were collected from the tumor-supply group 10 days after LLC injection and then injected subcutaneously into all of the 80 mice to establish the recurrent and metastatic mouse models of lung cancer. Subsequently,corresponding different substances were administrated intraperitoneally in the different treated groups since the next day. After 15 days’ administration,all the mice were sacrificed by cervical spine dislocation. Morphological characteristics were observed by H&E staining,and the protein expression of CD44,CD62 P and OPN were measured by immunohistochemistry. Results Compared with the model group,the pathological changes of the recurrent tissues in each treatment group were alleviated to some extent,especially in the DDP combined with200 μg/m L APS group;the expression of CD44,CD62 P and OPN in each treatment group decreased,especially in the DDP group and DDP combined with 100 and 200 μg/m L APS-treated groups. Conclusion APS combined with DDP could significantly inhibit the growth and metastasis of Lewis lung cancer cells,which might be associated with the reduced expression of CD44,CD62 P and OPN.