文献类型：期刊文献

英文题名：Impact of Agaricus blazei Murill Extract Combined With Imatinib Treatment on the Proliferation and Apoptosis of Multidrug-Resistant Leukemia Cells

作者：Wang, Dongping[1,2];Ge, Wanwen[3];Sun, Yanqing[1,2]

第一作者：Wang, Dongping

通信作者：Sun, YQ[1];Sun, YQ[2]

机构：[1]Gansu Univ Chinese Med, Sch Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;[2]Gansu Prov Hosp, Clin Educ Dept, Lanzhou 730000, Peoples R China;[3]Lanzhou Univ, Cuiying Biomed Res Ctr, Hosp 2, Lanzhou 730030, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Tradit Chinese & Western Med, Lanzhou 730000, Peoples R China;[2]corresponding author), Gansu Prov Hosp, Clin Educ Dept, Lanzhou 730000, Peoples R China.|[10735]甘肃中医药大学;

年份：2024

卷号：2024

外文期刊名：JOURNAL OF FOOD BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001350795200002)】；

基金：This work was supported by the Natural Science Foundation of Gansu Province (No. 20JR10RA376, 21JR11RA196, and 22JR5RA981), Lanzhou Science and Technology Plan Project (No. 2020-ZD-56), Cuiying Scientific and Technological Innovation Program of Lanzhou University Second Hospital (No. CY-2019-QN15), and Innovation Fund Project of Colleges and Universities in Gansu Province in 2020 (No. 2020B-040).

语种：英文

外文关键词：Agaricus blazei Murill; apoptosis; chronic myeloid leukemia; imatinib; PI3K/AKT/mTOR signaling

摘要：Multidrug resistance (MDR) is a major cause of chronic myeloid leukemia (CML) relapse, therapeutic failure, and a poor prognosis. However, Agaricus blazei Murill (AbM) is a mushroom that might have anticancer and other medicinal properties. Therefore, this study aimed to determine the effects of combining the acidic RNA protein complex FA-2-b-beta extracted from AbM with imatinib (IM) on MDR in the K562/ADR leukemia cell line in vitro and in xenograft mouse models. The combination of FA-2-b-beta and IM significantly inhibited cell proliferation and promoted apoptosis in K562/ADR cells compared with either alone. Western blotting (WB) revealed that the combination significantly reduced p-PI3K, p-AKT, and p-mTOR protein expression. The combination also inhibited tumor growth in mice with K562/ADR xenografts. These findings suggested that FA-2-b-beta enhances the effects of IM on K562/ADR cell proliferation and apoptosis, potentially by inhibiting the PI3K/AKT/mTOR pathway and downregulating P-glycoprotein (P-gp) expression.