文献类型：期刊文献

英文题名：Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier

作者：Dong, Shuqing[1,2];Gao, Ruibin[3];Yang, Yan[4];Guo, Mei[3];Ni, Jingman[4];Zhao, Liang[1,2]

第一作者：Dong, Shuqing

通信作者：Zhao, L[1]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Gansu Coll Tradit Chinese Med, Dept Pharm, Coll Gansu Prov, Key Lab Chem & Qual Tradit Chinese Med, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China.

年份：2014

卷号：449

期号：1

起止页码：158

外文期刊名：ANALYTICAL BIOCHEMISTRY

收录：;EI(收录号：20201908624675);Scopus(收录号：2-s2.0-84893187524);WOS:【SCI-EXPANDED(收录号:WOS:000332816100023)】；

基金：The authors acknowledge financial support from the National Natural Science Foundation of China (20675085), Science and Technology Support Xinjiang Project (XBXJ-2011-021), and Science and Technology Development Projects of Lanzhou (2010-1-156).

语种：英文

外文关键词：Capillary electrophoresis; Phenylethanoid glycosides and aglycones; beta-Cyclodextrin buffer modifier; Lamiophlomis rotata; Cistanche

摘要：Although the separation efficiency of capillary electrophoresis (CE) is much higher than that of other chromatographic methods, it is sometimes difficult to adequately separate the complex ingredients in biological samples. This article describes how one effective and simple way to develop the separation efficiency in CE is to add some modifiers to the running buffer. The suitable running buffer modifier beta-cyclodextrin (beta-CD) was explored to fast and completely separate four phenylethanoid glycosides and aglycones (homovanillyl alcohol, hydroxytyrosol, 3,4-dimethoxycinnamic acid, and caffeic acid) in Lamiophlomis rotata (Lr) and Cistanche by capillary zone electrophoresis with ultraviolet (UV) detection. It was found that when beta-CD was used as running buffer modifier, a baseline separation of the four analytes could be accomplished in less than 20 min and the detection limits were as low as 10(-3) mg L-1. Other factors affecting the CE separation, such as working potential, pH value and ionic strength of running buffer, separation voltage, and sample injection time, were investigated extensively. Under the optimal conditions, a successful practical application on the determination of Lr and Cistanche samples confirmed the validity and practicability of this method. (C) 2014 Elsevier Inc. All rights reserved.