文献类型：期刊文献

中文题名：白花蛇舌草总黄酮对BGC-823胃癌细胞增殖和凋亡的影响

英文题名：Effect of the total flavones of oldenlandia diffusa on the proliferation and apoptosis of gastric cancer cell BGC- 823

作者：王秋兰[1];高晓民[2];张煦[2]

第一作者：王秋兰

机构：[1]甘肃中医药大学临床医学院,甘肃兰州730000;[2]兰州大学病理研究所,甘肃兰州730000

第一机构：甘肃中医药大学临床医学院

年份：2015

卷号：24

期号：21

起止页码：2289

中文期刊名：现代中西医结合杂志

外文期刊名：Modern Journal of Integrated Traditional Chinese and Western Medicine

收录：CSTPCD

基金：甘肃省高等学校基本科研业务费项目(2012-5);甘肃省自然科学基金项目(145RJZA235)

语种：中文

中文关键词：白花蛇舌草;总黄酮;BGC-823胃癌细胞;增殖;凋亡

外文关键词：oldenlandia diffusa; total flavones; gastric cancer cells BGC -823; proliferation; apoptosis

摘要：目的探讨白花蛇舌草总黄酮对BGC-823胃癌细胞增殖和凋亡的影响。方法采用MTT法检测3.125,6.25,9.375,12.5,25.0及50.0μg/m L白花蛇舌草总黄酮作用24 h及48 h对BGC-823胃癌细胞的抑制率,流式细胞仪检测白花蛇舌草总黄酮对细胞周期及细胞凋亡的影响。结果作用24 h,白花蛇舌草总黄酮3.125,6.25μg/m L对BGC-823胃癌细胞无明显抑制作用,9.375,12.5,25,50μg/m L有明显抑制作用,其中12.5μg/m L抑制率最高为51.5%,同期1μg/m L顺铂组为82.4%;作用48 h,白花蛇舌草总黄酮3.125,50μg/m L对BGC-823胃癌细胞无明显抑制作用,6.25,9.375,12.5,25μg/m L对BGC-823胃癌细胞均有明显抑制作用,其中12.5μg/m L抑制率最高为67.3%,同期1μg/m L顺铂组为85.4%。实验各组48 h抑制率均高于24 h。经白花蛇舌草总黄酮梯度药物处理的BGC-823胃癌细胞悬浮死亡,且随药物浓度的增加悬浮死亡的细胞增多,细胞呈现凋亡特征。白花蛇舌草总黄酮试验组与对照组比较,G1期细胞增加,S期细胞明显减少,凋亡细胞比例明显增高。结论白花蛇舌草总黄酮对BGC-823胃癌细胞的生长增殖有明显抑制作用,并表现出一定范围内的时-效及量-效关系,作用机制可能与抑制DNA的合成、诱导细胞凋亡有关。
Objective It is to approach the influence of the total flavones of oldenlandia diffusa （TFOD） on the prolifera- tion and apoptosis of gastric cancer cell BGC - 823. Methods The inhibition of TFOD in dose of 3. 125, 6.25, 9. 375, 12.5, 25.0 and 50.0 pLg/mL on BGC - 823 cells for 24 and 48 hours were measured by MTT assay; the influence of TFOD on the proliferation cycle and apoptosis of BGC - 823 was detected by Flow Cytometry. Results After 24 hours, the dose of TFOD in 3. 125 and 6.25 μg/mL had no obvious inhibition, but in 9. 375, 12.5, 25.0 and 50.0 μg/mL had obvious inhibitory effect, the highest inhibition rate was 51.5% that appeared in 12.5 μg/mL group, positive control group 1μg/mL cisplatin was 82. 4% ; after 48 hours, the dose of TFOD in 3. 125 and 50.0 μg/mL had no obvious inhibition, but in 9. 375, 12.5 and 25.0 μg/mL had obvious inhibitory effect, the highest inhibition rate was 67.3% that appeared in 12.5 μg/mL group, too, positive control group 1 μg/mL cisplatin was 85.4% ; inhibition rate of 48 hours were higher than that of 24 hours in experimental group; BGC - 823 ceils treated by TFOD in gradient, cell died, and with the increase of drug concentration, the in- crease in the number of cells death; cell membrane shrinked, arranged sparsely, volume contracted, cell deformed into circular or elliptic, presented apoptosis features; compared to control group, cells in Gl phase of TFOD increased, and cells in S phase decreased significantly ; the apoptosis cell ratio is significantly higher than that of the control group. Conclusion TFOD has inhibitory effect on gastric cancer cells BGC - 823 in vitro and shows time-effect and dose-effect relationship in a certain range; the mechanism may he related to inhibition of TFOD in DNA synthesis and inducing cell apoptosis.