文献类型：期刊文献

中文题名：野生与栽培当归遗传多样性比较

英文题名：Comparative study on populations genetic diversity between wild and cultivated Angelica sinensis

作者：朱田田[1,2,3,4];晋玲[1,2,3];黄得栋[1,3];卢有媛[1,3];李金田[1];孙少伯[1]

第一作者：朱田田

机构：[1]甘肃中医药大学;[2]甘肃省中药质量与标准研究重点实验室培育基地;[3]甘肃中医药大学中(藏)药资源研究所;[4]甘肃中医药大学药用植物遗传育种研究所

第一机构：甘肃中医药大学

年份：2018

卷号：49

期号：1

起止页码：211

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81360615);甘肃省科技计划自然科学基金资助项目(1308RJZA127);甘肃省科技计划基础研究创新群体(1606RJIA323);中央财政引导地方科技创新平台项目"甘肃省中药资源与新产品开发平台建设"

语种：中文

中文关键词：当归;野生;栽培;遗传多样性;ISSR

外文关键词：Angelica sinensis （Oliv.） Diels; wildness; cultivation; genetic diversity; ISSR

摘要：目的比较野生当归Angelica sinensis与栽培当归的遗传多样性。方法应用ISSR分子标记对48个栽培当归居群和7个野生当归居群1 038个样本进行扩增,利用Popgen 1.32软件对其Shannon’s多样性信息指数(I)等遗传信息参数进行分析,并利用SPSS 19.0中的独立样本t检验进行差异显著性检验;运用Gen Al Ex 6.5软件对野生和栽培当归2个组群遗传变异进行AMOVA分析;应用NTSYS软件构建当归野生和栽培各居群的Nei’s遗传距离UPGMA聚类树状图,并使用Gen Al Ex6.5软件中的Mental test分析遗传距离与地理距离的相关性。结果野生当归居群和栽培当归居群的多态位点百分率(PPB)为83.77%和96.10%,I平均值为0.221 7和0.282 8,Nei’s基因多样性指数(H)平均值为0.344 4和0.434 3,且存在极显著差异(P<0.001);野生和栽培当归居群的种群间遗传分化指数(Gst)、基因流(Nm)值分别为0.299 1、1.171 8和0.733 4、0.181 7,组间变异百分比为15.85%,居群间变异百分比为54.3%,居群内变异百分比为29.85%;遗传距离变化范围0.014 4~0.730 7。结论当归野生居群的遗传多样性低于栽培居群,野生居群间的遗传分化程度明显低于栽培居群,当归总的遗传变异来自于居群间,野生居群与栽培居群亲缘关系较远,且遗传距离与地理距离存在相关性。
Objective To compare the populations genetic diversity between wild and cultivated populations of Angelica sinensis. Methods ISSR markers were used to analyze the genetic diversity of 1 038 samples, which included 48 cultivated populations and seven wild populations. Shannon＇s information index（I） and other parameters of genetic information were calculated by Popgen 1.32. Using independent sample t-test in SPSS19.0 to examine whether there was significant difference. The AMOVA analysis of the genetic variation of two groups was carried out by using Gen Al Ex 6.5; UPGMA dendrogram relationship between wild and cultivated populations were clustered by Ntsys, and the correlation of genetic distance and geographic distance was analyzed by using the mental test in Gen Al Ex 6.5. Results The average percentages of polymorphie bands（PPB） of wild and cultivated populations were 83.77% and 96.10%; Shannon＇s information indexes（I） were 0.221 7 and 0.282 8; The average values of Nei＇s genetic diversity index（He） were 0.344 4 and 0.434 30, and there had extremely significant differences（P〈0.001）. The genetic differentiation coefficient（Gst） and gene flow（Nm） of wild populations were 0.299 1 and 1.171 8, which of cultivated populations were 0.733 4 and 0.181 7. The variation percentage was 15.85% between groups of wild and cultivated. The variation percentage among populations was 54.3%, and the variation percentage within populations was 54.3%; The genetic distance varied from 0.014 4 to 0.730 7. Conclusion The genetic diversity of wild populations was lower than the cultivated populations; The level of genetic differentiation among wild populations was obviously lower than the cultivated populations; The total genetic variation of A. sinensis mainly existed among populations; It has a distant relationship between wild and cultivated populations, and has correlation between genetic distance and geographic distance.