文献类型：期刊文献

中文题名：抗病促生细菌的筛选及叶面喷施复配菌剂对当归质量的影响

英文题名：Screening of disease-resistant and plant growth-promoting bacteria and evaluation of the effects of foliar spraying with compound microbial agents on the quality of Angelica sinensis

作者：杜晓雪[1,2];杨涛[1,3];谢田朋[1,2];丁琦[1,2];杨林桦[1,2];黄钰芳[1,2]

第一作者：杜晓雪

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]陇药产业创新研究院,甘肃兰州730000;[3]甘肃省科学院生物研究所甘肃省微生物资源开发利用重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：53

期号：2

起止页码：761

中文期刊名：微生物学通报

外文期刊名：Microbiology China

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金(82160714,82560755);甘肃省青年人才项目(2024QNTD36);甘肃省自然科学基金(23JRRA1711);兰州市指导性计划(2022-5-167);甘肃省高校教师创新基金(2025A-101);甘肃省中药质量与标准研究重点实验室开放基金(ZYZL-2024-04);甘肃省科学院产业化项目(2024YLCY-01);平凉市科技局引导计划(PL-STK-2023A-061)。

语种：中文

中文关键词：当归;叶面喷施;芽孢杆菌属;短芽孢杆菌属;质量

外文关键词：Angelica sinensis;foliar spraying;Bacillus;Brevibacillus;quality

摘要：背景连作障碍严重影响了当归种植产业的健康发展,开发能够提升当归质量的复合菌剂已成为当务之急。目的研究叶面喷施抗病和不抗病2类复合菌剂对连作当归质量的影响,为研发更有效的抗病促生复合菌剂提供理论依据和参考。方法采用稀释平板涂布分离法分离菌株;显微形态观察结合分子鉴定技术鉴定菌株;采用常规方法测定菌株生理生化指标,并统计当归生长、病害情况;采用高效液相色谱法测定当归根中有效成分含量;试剂盒检测法测定植物生理生化指标及菌株相关酶活性;采用超高效液相色谱串联质谱联用技术结合多反应监测模式检测内源激素代谢物含量。结果共筛选出6株对当归具有一定促生功能的菌株,根据对茄腐镰刀菌(Fusarium solani)的拮抗作用分为由贝莱斯芽孢杆菌(Bacillus velezensis)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)与萎缩芽孢杆菌(Bacillus atrophaeus)组成的抗病菌剂(KJ)和侧孢短芽孢杆菌(Brevibacillus laterosporus)、耐寒短芽孢杆菌(Peribacillus frigoritolerans)、巨大芽孢杆菌(Bacillus megaterium)组成的无拮抗活性菌剂(BJ),并将菌株复配后开展田间叶面喷施试验。KJ组和BJ组均促进了当归生长、缓解了土壤病害,有利于当归有效成分的积累:较对照组(CK),KJ组百株重增加29.69%、根腐病发病率降低69.44%(P<0.05);BJ组百株重增加47.97%、根腐病发病率降低25.00%(P<0.05)。KJ组显著提高了叶片丙二醛含量、亚硝酸还原酶活性、铵态氮、净光合速率(net photosynthetic rate,Pn)及蒸腾速率(transpiration rate,Tr),显著降低了DPPH清除率及硝酸还原酶、谷氨酸合成酶、可溶性糖、叶绿素(P<0.05);BJ组显著提高了叶片丙二醛、过氧化物酶、总酚、类黄酮、谷氨酰胺合酶、铵态氮及Pn、Tr(P<0.05)。此外,KJ组较CK组显著上调了细胞分裂素、水杨酸类激素水平,下调了茉莉酸、赤霉素类激素水平;BJ组显著上调了脱落酸、生长素、细胞分裂素、赤霉素类激素水平及12-氧代植物二烯酸。结论由贝莱斯芽孢杆菌、解淀粉芽孢杆菌与萎缩芽孢杆菌组成的抗病菌剂(KJ)和侧孢短芽孢杆菌、耐寒短芽孢杆菌、巨大芽孢杆菌组成的无抑菌活性菌剂(BJ)均对当归具有抗病、促生作用。抗病菌剂能够在镰刀菌病原菌胁迫环境下高效防控病害,但同时也需付出氧化毒性代价;不抗病菌剂可能更适合多种复杂环境,通过优化当归自身生理功能,促进植株生长与防御潜能提升。
Background Continuous cropping obstacles have severely hindered the healthy development of the Angelica sinensis cultivation industry,making the development of compound microbial agents to enhance the quality of this medicinal plant an urgent priority.Objective We investigated the effects of foliar spraying with disease resistant and non-disease-resistant composite microbial agents on the quality of A.sinensis under continuous cropping conditions,aiming to provide a theoretical basis for developing more effective disease-resistant and growth-promoting compound microbial agents.Methods Bacterial strains were isolated via the dilution plate coating method and identified through morphological observation combined with molecular techniques.Conventional methods were employed to determine the physiological and biochemical characteristics of the strains,as well as to evaluate the growth and disease incidence of A.sinensis.The content of bioactive compounds in A.sinensis roots was quantified by HPLC.Commercial assay kits were used to measure the plant physiological and biochemical parameters,along with strain-related enzyme activities.Endogenous hormone metabolites were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry with multiple reaction monitoring(UPLC-MS/MS+MRM).Results A total of six bacterial strains exhibiting growth-promoting effects on A.sinensis were screened out.According to their antagonistic activity against Fusarium solani,they were classified into two groups:the antagonistic strain(KJ)group consisting of Bacillus velezensis,B.amyloliquefaciens,and B.atrophaeus and the non-antagonistic strain(BJ)group comprising Brevibacillus laterosporus,Peribacillus frigoritolerans,and B.megaterium.Field foliar spraying experiments were conducted with the compound agents developed with these strains.Both KJ and BJ treatments promoted A.sinensis growth,alleviated soil-borne diseases,and enhanced the accumulation of active components.Compared with the control(CK),the KJ treatment increased the fresh weight per 100 plants by 29.69%and reduced the root rot incidence by 69.44%(P<0.05).The BJ treatment increased the fresh weight per 100 plants by 47.97%and reduced the root rot incidence by 25.00%(P<0.05).Compared with CK,the KJ treatment increased the malondialdehyde(MDA),nitrite reductase,ammonium nitrogen,net photosynthetic rate(Pn),and transpiration rate(Tr),but decreased the DPPH radical scavenging activity,nitrate reductase,glutamine synthetase,soluble sugars,and chlorophyll content of leaves(P<0.05).The BJ treatment enhanced the MDA,peroxidase activity,total phenols,flavonoids,glutamine synthetase,ammonium nitrogen,Pn,and Tr of leaves(P<0.05).Additionally,the KJ treatment significantly raised the cytokinin and salicylic acid levels while lowering the jasmonic acid and gibberellin levels compared with CK.The BJ treatment significantly elevated the abscisic acid,auxin,cytokinin,gibberellin,and 12-oxo-phytodienoic acid levels.Conclusion Both the KJ agent composed of B.velezensis,B.amyloliquefaciens,and B.atrophaeus and the BJ agent consisting of B.laterosporus,Peribacillus frigoritolerans,and B.megaterium demonstrate disease-resistant and growth-promoting effects on A.sinensis.The KJ agent effectively controls diseases under Fusarium stress but incurs oxidative toxicity costs.The KJ agent may be more adaptable to complex environments and enhances plant growth and defense potential by optimizing the physiological functions of A.sinensis.