文献类型：期刊文献

英文题名：Recovery of motor function in rats with complete spinal cord injury following implantation of collagen/silk fibroin scaffold combined with human umbilical cord-mesenchymal stem cells

作者：Deng, Wu-Sheng[1];Liu, Xiao-Yin[2,3];Ma, Ke[2];Liang, Bing[2];Liu, Ying-Fu[4];Wang, Ren-Jie[2];Chen, Xu-Yi[2];Zhang, Sai[2]

第一作者：Deng, Wu-Sheng

通信作者：Zhang, S[1]

机构：[1]Gansu Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Lanzhou, Gansu, Peoples R China;[2]Chinese Peoples Armed Police, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Characterist Med Ctr, Tianjin, Peoples R China;[3]Sichuan Univ, West China Hosp, Dept Neurosurg, Chengdu, Peoples R China;[4]Cangzhou Nanobody Technol Innovat Ctr, Cangzhou, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Chinese Peoples Armed Police, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Characterist Med Ctr, Tianjin, Peoples R China.

年份：2021

卷号：67

期号：9

起止页码：1342

外文期刊名：REVISTA DA ASSOCIACAO MEDICA BRASILEIRA

收录：;Scopus(收录号：2-s2.0-85120075386);WOS:【SCI-EXPANDED(收录号:WOS:000720928800027)】；

基金：Funding: National Natural Science Foundation of China.

语种：英文

外文关键词：Rats; Collagen; Silk fibroin; human mesenchymal stem cells; Nerve regeneration; Spinal cord injuries

摘要：OBJECTIVE: This study aimed to assess the effect of the collagen/silk fibroin scaffolds seeded with human umbilical cord-mesenchymal stem cells on functional recovery after acute complete spinal cord injury. METHODS: The fibroin and collagen were mixed (mass ratio, 3:7), and the composite scaffolds were produced. Forty rats were randomly divided into the Sham group (without spinal cord injury), spinal cord injury group (spinal cord transection without any implantation), collagen/silk fibroin scaffolds group (spinal cord transection with implantation of the collagen/silk fibroin scaffolds), and collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (spinal cord transection with the implantation of the collagen/ silk fibroin scaffolds co-cultured with human umbilical cord-mesenchymal stem cells). Motor evoked potential, Basso-Beattie-Bresnahan scale, modified Bielschowsky's silver staining, and immunofluorescence staining were performed. RESULTS: The BBB scores in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group were significantly higher than those in the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). The amplitude and latency were markedly improved in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.05 or p<0.01). Meanwhile, compared to the spinal cord injury and collagen/silk fibroin scaffolds groups, more neurofilament positive nerve fiber ensheathed by myelin basic protein positive structure at the injury site were observed in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group (p<0.01, p<0.05). The results of Bielschowsky's silver staining indicated more nerve fibers was observed at the lesion site in the collagen/silk fibroin scaffolds + human umbilical cord-mesenchymal stem cells group compared with the spinal cord injury and collagen/silk fibroin scaffolds groups (p<0.01, p< 0.05). CONCLUSION: The results demonstrated that the transplantation of human umbilical cord-mesenchymal stem cells on a collagen/silk fibroin scaffolds could promote nerve regeneration, and recovery of neurological function after acute spinal cord injury.