文献类型：期刊文献

英文题名：Identification of Differentially Expressed mRNAs and lncRNAs Contributes to Elucidation of Underlying Pathogenesis and Therapeutic Strategy of Recurrent Implantation Failure

作者：Liu, Lin[1,2,3,4];Liu, Yidan[2];Tian, Yu[1];Cao, Ying[5];Wang, Ting[1];Mi, Shengyan[1];Yang, Run[6];Liu, Simin[6];Ma, Xiaoling[1,2,3,4];Wang, Jing[6]

第一作者：Liu, Lin

通信作者：Liu, L[1];Ma, XL[1];Liu, L[2];Ma, XL[2];Liu, L[3];Ma, XL[3];Liu, L[4];Ma, XL[4];Wang, J[5]

机构：[1]Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China;[2]Lanzhou Univ, Basic Med Sci Coll, Lanzhou, Gansu, Peoples R China;[3]First Hosp Lanzhou Univ, Reprod Ctr, Lanzhou, Gansu, Peoples R China;[4]Clin Res Ctr Reprod Dis Gansu Prov, Lanzhou, Gansu, Peoples R China;[5]Wuhan Univ, Reprod Med Ctr, Renmin Hosp, Wuhan 430060, Hubei, Peoples R China;[6]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Clin Med Coll 1, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Basic Med Sci Coll, Lanzhou, Gansu, Peoples R China;[3]corresponding author), First Hosp Lanzhou Univ, Reprod Ctr, Lanzhou, Gansu, Peoples R China;[4]corresponding author), Clin Res Ctr Reprod Dis Gansu Prov, Lanzhou, Gansu, Peoples R China;[5]corresponding author), Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou, Gansu, Peoples R China.|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2024

外文期刊名：REPRODUCTIVE SCIENCES

收录：;Scopus(收录号：2-s2.0-85197368401);WOS:【SCI-EXPANDED(收录号:WOS:001261430700001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (82360307), the Talent Innovation and Entrepreneurship Project of Lanzhou City (2022-RC-47), Gansu Provincial Science and Technology Program (20JR10TA715, 21JR7RA391), the Medical Innovation and Development Project of Lanzhou University (lzuyxcx-2022-191), Science and Technology Pro-ject of Lanzhou (2023-ZD-93), Fund of the First Hospital of Lanzhou University (ldyyyn2022-60), the First Hospital of Lanzhou University Excellence Program (20220060074, 20220060073).

语种：英文

外文关键词：Recurrent implantation failure; Messenger RNA; Long non-coding RNA; Competing endogenous RNA; Endometrial receptivity; ML-193

摘要：Recurrent implantation failure (RIF) is a complex and poorly understood clinical disorder characterized by failure to conceive after repeated embryo transfers. Endometrial receptivity (ER) is a prerequisite for implantation, and ER disorders are associated with RIF. However, little is known regarding the molecular mechanisms underlying ER in RIF. In the present study, RNA sequencing data from the mid-secretory endometrium of patients with and without RIF were analyzed to explore the potential long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) involved in RIF. The analysis revealed 213 and 1485 differentially expressed mRNAs and lncRNAs, respectively (fold change >= 2 and p < 0.05). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that these genes were mostly involved in processes related to immunity or inflammation. 5 key genes (TTR, ALB, TF, AFP, and CFTR) and a key module including 14 hub genes (AFP, ALB, APOA1, APOA2, APOB, APOH, FABP1, FGA, FGG, GC, ITIH2, SERPIND1, TF and TTR) were identified in the protein-protein interaction (PPI) network. The 5 key genes were used to further explore the lncRNA-miRNA-mRNA regulatory network. Finally, the drug ML-193 based on the 14 hub genes was identifed through the CMap. After ML-193 treatment, endometrial cell proliferation was increased, the hub genes were mostly down-regulated, and the ER marker HOXA10 was up-regulated. These results offer insights into the regulatory mechanisms of lncRNAs and mRNAs and suggest ML-193 as a therapeutic agent for RIF by enhancing ER.