文献类型：期刊文献

英文题名：Bioinformatic analysis of the role of immune checkpoint genes and immune infiltration in the pathogenesis and development of premature ovarian insufficiency

作者：Zhang, Xiyan[1];Wang, Ling[1];Yang, Tongkun[2];Kong, Li[1,3];Wei, Luxiao[1,3];Du, Jing[1,3]

第一作者：Zhang, Xiyan

通信作者：Wang, L[1]

机构：[1]940 Hosp Joint Logist Support Force Chinese People, Lanzhou 730050, Gansu, Peoples R China;[2]Peoples Liberat Army Gen Hosp, Dept Obstet & Gynecol, Med Ctr Chinese 1, Beijing 100039, Peoples R China;[3]Gansu Univ Chinese Med, Lanzhou 730030, Gansu, Peoples R China

第一机构：940 Hosp Joint Logist Support Force Chinese People, Lanzhou 730050, Gansu, Peoples R China

通信机构：[1]corresponding author), 940 Hosp Joint Logist Support Force Chinese People, Lanzhou 730050, Gansu, Peoples R China.

年份：2024

外文期刊名：JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

收录：;Scopus(收录号：2-s2.0-85191971733);WOS:【SCI-EXPANDED(收录号:WOS:001216953200002)】；

基金：No Statement Available

语种：英文

外文关键词：Gene Expression Omnibus database; Premature Ovarian Insufficiency; Immune checkpoint genes; Bioinformatics

摘要：Purpose With advances in immunology, increasing evidence suggests that immunity is involved in premature ovarian insufficiency (POI) pathogenesis. This study investigated the roles of immune checkpoint genes and immune cell infiltration in POI pathogenesis and development.Methods The GSE39501 dataset and immune checkpoint genes were obtained from the Gene Expression Omnibus database and related literature. The two datasets were intersected to obtain immune checkpoint-related differentially expressed genes (ICRDEGs), which were analyzed using Gene Ontology and Kyoto Encyclopedia of Gene and Genomes enrichment analysis, weighted correlation network analysis, protein-protein interaction and related microRNAs, transcription factors, and RNA binding proteins. The immune cell infiltration of ICRDEGs was explored, and receiver operating characteristic curves were used to validate the diagnostic value of ICRDEGs in POI.Results We performed ICRDEG functional enrichment analysis and found that these genes were closely related to immune processes, such as T cell activation. Specifically, they are enriched in various biological processes and pathways, such as cell adhesion molecule and T cell receptor signaling pathways. Weighted correlation network analysis identified seven hub genes: Cd200, Cd274, Cd28, neurociliary protein-1, Cd276, Cd40lg, and Cd47. Furthermore, we identified 112 microRNAs, 17 RNA-binding proteins, and 101 transcription factors. Finally, immune infiltration analysis showed a clear positive correlation between hub genes and multiple immune cell types.Conclusion Bioinformatic analysis identified seven potential ICRDEGs associated with POI, among which the immune checkpoint molecules CD200 and neurociliary protein-1 may be involved in the pathogenesis of POI.