文献类型：期刊文献

中文题名：高原低氧环境气虚小鼠心、肺反应性变化的分子机制研究

英文题名：Study on Molecular Mechanism of Reactive Changes in Heart and Lung of Mice in High Altitude Hypoxia

作者：骆亚莉[1,2];刘永琦[1,2];安方玉[3];孙丽姣[4];蔡路路[4];李欣[4];马彦平[4]

第一作者：骆亚莉

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室;[2]敦煌医学与转化省部共建教育部重点实验室;[3]甘肃中医药大学教学实验中心;[4]甘肃中医药大学公共卫生学院

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2015

卷号：22

期号：12

起止页码：50

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2015_2016】；

基金：甘肃省科技支撑计划(1204FKCA169);国家科技支撑计划(2011BAI05B02);敦煌医学与转化省部共建教育部重点实验室开放基金(DHYX1213-012)

语种：中文

中文关键词：高原低氧暴露;气虚;乳酸脱氢酶;钠-钾-ATP酶;缺氧诱导因子-1α;水通道蛋白-5;小鼠

外文关键词：high altitude hypoxia exposure;;qi deficiency;;LDH;;Na+-K+-ATPase;;HIF-1α;;AQP-5;;mice

摘要：目的探讨高原低氧环境气虚小鼠心、肺反应性变化的分子机制。方法 SPF级模型组小鼠置于低氧舱内进行减压低氧暴露,对照组在常氧环境中饲养,连续21 d。观察小鼠体征。末次低氧暴露后检测2组小鼠心乳酸脱氢酶(LDH)、Na+-K+-ATP酶活性,肺功能变化,心、肺组织缺氧诱导因子-1α(HIF-1α)及肺组织通道蛋白-5(AQP5)蛋白和基因表达。结果模型组小鼠出现气虚体征。与对照组比较,模型组小鼠心LDH活性增高、Na+-K+-ATP酶活性降低(P<0.05,P<0.01);肺功能指标检测结果显示,模型组小鼠吸气时间、呼气时间、持续时间、潮气量、呼气末端停顿均降低和频率升高(P<0.05,P<0.01);心、肺HIF-1α蛋白、基因表达均增高,肺AQP-5基因表达增高(P<0.05,P<0.01)。结论较长时间的高原低氧暴露,可导致小鼠表现气虚体征并出现肺通气效率降低。心肌受损可能与心Na+-K+-ATP酶活性变化有关,而心、肺HIF-1α表达及肺AQP-5基因表达升高,可能有利于提高心、肺的适应性代偿反应。
Objective To discuss the molecular mechanism of reactive changes in heart and lung of mice models in high altitude hypoxia environment. Methods Healthy SPF mice were put in hypoxia cabin for decompression and hypoxia exposure to make model, and mice in the control group were fed in normoxia environment for 21 days. The physical signs of the mice were observed. After the last time of hypoxia exposure, mice were detected for indexes of correlation along with the control group. LDH, Na+-K+-ATPase activities of heart, changes of pulmonary function, and gene and protein expression of HIF-1α in the heart and lung tissue, AQP-5 in the lung tissue were detected. Results Mice in the model group showed signs of qi deficiency. Compared with the control group, Na+-K+-ATPase activity of heart tissue was lowered(P <0.01) but LDH was raised significantly(P <0.05) in the model group. The detection results of pulmonary function displayed that the indexes such as Ti, Te, RT, EEP and TV were all dropped(P <0.05 or P <0.01) in the model group. However, the respiratory frequency increased significantly. The gene and protein expressions of HIF-1α in heart and lung all increased(P <0.05 or P <0.01) in the model group. Also the gene expression of AQP-5 in lung increased obviously(P <0.01). Conclusion Long time exposure in high altitude hypoxia environment can cause qi deficiency and low pulmonary ventilation. Impaired myocardium may be related to the changes of Na+-K+-ATPase activity. The increase of expressions of HIF-1α in heart and lung and AQP-5 in lung may be beneficial to adaptive compensatory reaction of heart and lung.