文献类型：期刊文献

英文题名：Integrative Analysis of Long Non-Coding RNAs (lncRNAs), miRNAs, and mRNA-Associated ceRNA Network in Lung Tissue of Aging Mice and Changes After Treatment with Codonopsis pilosula

作者：Chen, Dongmei[1];Liu, Jiajia[1];Meng, Jie[1];Li, Dandan[1];Zhao, Pan[1];Duan, Yongqiang[2];Wang, Jing[1,3]

第一作者：Chen, Dongmei

通信作者：Wang, J[1];Wang, J[2]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Sch Basic Med, Lanzhou, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Key Lab Tradit Chinese Herbs & Prescript Innovat, Lanzhou, Gansu, Peoples R China

第一机构：甘肃中医药大学临床医学院

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Key Lab Tradit Chinese Herbs & Prescript Innovat, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;[10735c1ddf597548aedb5]甘肃中医药大学临床医学院;

年份：2020

卷号：26

外文期刊名：MEDICAL SCIENCE MONITOR

收录：;Scopus(收录号：2-s2.0-85079335626);WOS:【SCI-EXPANDED(收录号:WOS:000513534000001)】；

基金：This research was funded by the National Natural Science Foundation of China (grant number 81760835) and the General Project of Scientific Research in Colleges and Universities of Gansu Province (grant number 2018A-052)

语种：英文

外文关键词：Aging; Codonopsis; Lung Diseases; Microarray Analysis

摘要：Background: Codonopsis pilosula is a traditional Chinese medicine that has an anti-aging effect. However, the anti-aging effect of Codonopsis pilosula on the lungs remains largely unknown, and the molecular mechanism also needs to be further studied. Thus, we investigated the protective effect of Codonopsis pilosula on the lungs of aging mice, and explored the underlying molecular mechanism. Material/Methods: We established an aging mouse model and then treated the mice with Codonopsis pilosula. Microarray analysis and bioinformatics methods were used to comprehensively analyze the lncRNA-miRNA-mRNA (ceRNA) network. Results: Our results showed that we successfully established the aging mouse model. The microarray analysis showed that 138 lncRNAs, 128 mRNAs, and 7 miRNAs were significantly changed after aging, and 282 lncRNAs, 283 mRNAs, and 19 miRNAs were dysregulated after treatment with Codonopsis pilosula. To explore the signaling pathways involved, KEGG pathway analysis was performed. Compared with the ceRNA network in aging mice and after treatment with Codonopsis pilosula, we found that 3 mRNAs (Hif3a, Zbtb16, Plxna2) and 1 lncRNA (NONMMUT063872) were associated with the anti-aging effect of Codonopsis pilosula and they were validated by quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Conclusions: Our results showed that Codonopsis pilosula has a protective effect on the aging lung, and the ceRNA network plays an important role in the anti-aging effect of Codonopsis pilosula.