文献类型：期刊文献

中文题名：基于fNIRs探讨针刺干预单眼剥夺大鼠眼优势柱“漂移”的脑功能机制

英文题名：Discussion on mechanism of acupuncture for the ocular dominance'shift'in visual cortex of monocularly deprived rats based on fNIRs

作者：马重兵[1];贾静[1];孙燕[1];王觉[1];严兴科[1]

第一作者：马重兵

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2020

卷号：35

期号：5

起止页码：2259

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金项目(No.81860879,No.81660816,No.81260560);甘肃中医药大学中青年创新基金项目(No.ZQ2015-1)。

语种：中文

中文关键词：弱视;针刺;单眼剥夺;眼优势柱;功能近红外光谱技术;脑功能;机制

外文关键词：Amblyopia;Acupuncture;Monocular deprivation;Ocular dominance column;fNIRs;Brain function;Mechanism

摘要：目的:从眼优势柱"漂移"和重组角度探讨针灸干预弱视的脑功能机制。方法:70只SD大鼠随机分出空白组10只,剩余60只采用右眼缝合复制视觉剥夺模型。造模大鼠随机分为模型组,左旋多巴甲酯(LDME)组及早期、中期、末期针刺组。LDME组给予LDME灌胃,各针刺组均选取双侧睛明、攒竹、风池、光明,分别于模型复制后第3、12、21天开始针刺,1次/d,每次留针10min,共9d。干预结束后检测各组大鼠图形视觉诱发电位(P-VEP)改变,采用功能近红外光谱技术(fNIRs)检测静息态视皮层眼优势柱氧合血红蛋白(Oxy-Hb)、脱氧血红蛋白(Deoxy-Hb)、总血红蛋白(Total-Hb)浓度改变。结果:与空白组右眼P-VEP比较,模型组右眼潜伏期延长,振幅降低(P<0.05);与模型组右眼比较,LDME组、早期针刺组潜伏期缩短,各治疗组振幅升高(P<0.05);早期针刺组与LDME组P-VEP改善程度均优于中期、末期针刺组(P<0.05)。与空白组左侧眼优势柱Oxy-Hb浓度比较,模型组显著降低(P<0.01);与模型组比较,各治疗组显著升高(P<0.01),LDME组优于早期针刺组(P<0.01),早期针刺组优于中、末期针刺组(P<0.05)。结论:单眼(右眼)剥夺后对侧(左侧)视皮层眼优势柱功能显著抑制,同侧(右侧)眼优势柱功能异常重组;早期针刺能有效调节视觉剥夺后视功能柱"漂移"和重组现象,这为针灸干预视觉剥夺效应的脑功能机制提供了依据。
Objective: To explore the mechanism of the ocular dominance ‘shift’ of acupuncture against amblyopia. Methods: Seventy SD rats were randomly divided into the blank group(10 rats) and the rest(60 rats) were sutured with the right eye to duplicate the visual deprivation model. The 60 visually deprived rats were randomly assigned into the following 5 groups: model group, LDME group, early-stage acupuncture group, middle-stage acupuncture group and lately-stage acupuncture group, LDME group rats were intragastric administered of Levodopa Methyl Ester Hydrochloride, while the rats in the acupuncture groups were treated with acupuncture at bilateral Jingming(BL1), Cuanzhu(BL2), Fengchi(GB20) and Guangming(GB37) on the 3 rd, 12 th, and 21 st days, respectively after modeling, the needles were retained for 10 min and it was done once a day, for a total of 9 days. After treatment, the changes of P-VEP and the concentrations of Oxy-Hb, Deoxy-Hb and Total-Hb were detected by fNIRs. Results: After treatment, the latency of the right eye in model group was longer and the amplitude was lower than that in the blank group(P<0.05), and the latency of the right eye in LDME group and early-stage acupuncture group was shorter and the amplitude in treatment groups was higher than that in model group(P<0.05). P-VEP improvements in early-stage acupuncture group and LDME group was better than that in middle-stage acupuncture group and lately-stage acupuncture group(P<0.05). The concentration of Oxy-Hb in the ocular dominance column of left eye in model group was significantly lower than that in blank group(P<0.01). While compared with model group, it was significantly higher in treatment groups(P<0.01), among which the LDME group was higher than that in early-stage acupuncture group(P<0.01), and the early-stage acupuncture group was higher than that in middle-stage and late-stage acupuncture groups(P<0.05). Conclusion: The functions of the ocular dominance columns in rats’ contralateral(left) visual cortex are significantly inhibited after unilateral(right eye) deprivation, and the functions of the ocular dominance columns in rats’ ipsilateral(right) visual cortex are reorganized abnormally. Early acupuncture can effectively regulate the ‘shift’ and reorganization of visual column after visual deprivation, which provides a basis for the brain mechanism of acupuncture’s deprivation effect against amblyopia.