文献类型：期刊文献

中文题名：红芪多糖对局灶性脑缺血大鼠脑损伤的保护作用及对相关代谢物质的影响

英文题名：Protective effect and the influence on related metabolite substances of Hedysarum polybotrys polysaccharides in local cerebral cerebral ischemia of rats

作者：邵晶[1,2];吴国泰[1,3];王志旺[1,3];杜丽东[1,3];任远[1,4];郭玫[1,2]

第一作者：邵晶

机构：[1]甘肃中医药大学药学院,兰州730000;[2]甘肃中医药大学甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000;[3]甘肃中医药大学甘肃省中药药理与毒理学重点实验室,兰州730000;[4]甘肃中医药大学甘肃中医药大学甘肃省中药药理与毒理学重点实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：34

期号：4

起止页码：432

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金资助项目(17JR5RA163); 甘肃省教育厅科研基金资助项目(2013A-085); 甘肃省高等学校基本科研业务费基金资助项目(2013-1)

语种：中文

中文关键词：红芪多糖;局灶性脑缺血;神经功能;脑组织损伤

外文关键词：Hedysarum polybotrys polysaccharides;local cerebral cerebral ischemia; neurological function;brain tissue damage

摘要：目的研究红芪多糖(HPS)对局灶性脑缺血损伤大鼠脑组织神经功能、脑含水量、梗死面积比和脑组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、氧化产物丙二醛(MDA)与血清中一氧化氮(NO)、一氧化氮合成酶(NOS)、内皮素(ET)、降钙素基因相关肽(CGRP)的影响。方法按照体重将大鼠随机分为6组,每组10只:模型组,假手术组,对照组(银杏叶分散片,0.21 g·kg^(-1))与高、中、低3个剂量实验组(HPS 0.6,0.3,0.15 g·kg^(-1))。模型组、假手术组灌胃等量蒸馏水;几组均1天1次,连续7 d。第8天造模。用50%三氯化铁制备大鼠的大脑中动脉造局灶性脑缺血模型。用5级4分评分标准进行神经功能评分;用称重法检测脑含水量;用噻唑蓝法检测脑梗死面积比;用试剂盒检测脑组织中SOD、CAT、MDA以及以及血清中NO、NOS、ET、CGRP的含量。结果模型组与假手术组比较,所有指标差异均有统计学意义(均P<0.01)。给药后,模型组与高、中2个剂量实验组的脑组织中SOD(U·mg^(-1))分别为107.90±30.12,135.06±3.21,129.32±20.71;这3组的脑组织MDA(μmol·g^(-1))含量分别为6.39±2.03,4.08±1.54,4.53±1.21;这3组的NO(μmol·L^(-1))分别为66.32±20.39,48.50±20.86,52.39±27.01;这3组的NOS(U·mL^(-1))分别为29.95±8.05,21.94±9.98,20.55±8.06;这3组的ET(pg·mL^(-1))分别为96.64±21.51,75.88±19.52,76.42±17.76;这3组的CGRP(pg·mL^(-1))分别为53.98±10.95,76.43±22.53,67.07±12.12,组间的上述所有指标进行比较差异均有统计学意义(均P<0.05)。结论高、中2个剂量HPS对局灶性脑缺血大鼠模型神经功能损伤及脑组织损伤有一定保护作用;对局灶性脑缺血大鼠所造成的脑组织抗氧化系统的损伤有所改善。
Objective To study the effect of Hedysarum po-ybotrys polysaccharides（ HPS） on superoxide dismutase（ SOD）,catalase（ CAT）,maleic dialdehyde（ MDA）,nitric oxide（ NO）,nitric oxide synthetase（ NOS）,endothelin（ ET）,calcitonin generelated peptide（ CGRP） in local cerebral cerebral ischemia of rats. Methods The SPF degree Wister rats were randomly divided into six groups： model,sham orperation,control（ Ginkgo leafdispersible tablet,0. 21 g·kg^-1）,experimental-H,experimental-M,and experimental-L（ HPS,0. 60,0. 3,0. 15 g ·kg^-1）groups. Each group had ten rats. Local cerebral cerebral ischemia rat models were made by 50% Fe Cl3 on middle cerebral artery. Neurological score was made by blind method of level 5 and 4 points scoring method.Brain water content was detected by weight method. Brain infarction areawas detected by TTC staining method. The SOD,CAT,MDA of brain tissue and NO,NOS,ET,CGRP of serum were detected by kits. Results Compared with model group and sham operation group,all factors had significant difference（ all P〈0. 01）. After taking the medicine,the SOD（ U·mg^-1） in model,experimental-H and experimental-M groups were 107. 90 ± 30. 12,135. 06 ± 3. 21,129. 32 ± 20. 71; the MDA（ μmol·g^-1） in the three groups were 6. 39 ± 2. 03,4. 08 ± 1. 54,4. 53 ± 1. 21; the NO（ μmol · L^-1） in the three groups were 66. 32 ±20. 39,48. 50 ± 20. 86,52. 39 ± 27. 01; the NOS（ U · m L^-1） in the three groups were 29. 95 ± 8. 05,21. 94 ±9. 98,20. 55 ± 8. 06; the ET（ pg · m L^-1） in the three groups were 96. 64 ± 21. 51,75. 88 ± 19. 52,76. 42 ±17. 76; the CGRP（ pg · m L^-1） in the three groups were 53. 98 ± 10. 95,76. 43 ± 22. 53,67. 07 ± 12. 12,the differences of all above factors between groups were statistically significant（ all P〈0. 05）. Conclusion The experimental-H and experimental-M groups can protect nerve function injury and brain tissue injury and improve the damage of the brain tissue antioxidant system in the rat model of focal cerebral ischemia.