文献类型：期刊文献

中文题名：当归-红芪超滤膜提取物对糖尿病肾病大鼠肾组织TGF-β_1表达的影响

英文题名：Effect of Ultra-filtration Extract from Angelice Sinensis Radix and Hedysari Radix on TGF-β_1 in Kidney Tissue of Rat with Diabetic Nephropathy

作者：陈张敏[1];李荣科[1];张鹏飞[1];万生芳[1];张凌伟[2];郭钰龙[1];王秋兰[1]

第一作者：陈张敏

机构：[1]甘肃中医药大学,兰州730000;[2]石家庄市妇幼保健院,石家庄050000

第一机构：甘肃中医药大学

年份：2017

卷号：23

期号：5

起止页码：113

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目(81560718);甘肃省普通本科高等学校基本科研业务费项目[甘财教(2014)63号]

语种：中文

中文关键词：当归-红芪;超滤膜提取物;糖尿病肾病;转化生长因子-β1;Smad2/3

外文关键词：transforming growth Angelice Sinensis Radix-Hedysari Radix; ultra-filtration extract; diabetic nephropathy; factor-β1 ; Smad2/3

摘要：目的:研究当归-红芪超滤膜提取物对糖尿病肾病(DN)大鼠肾组织转化生长因子-β_1(TGF-β_1),Smad信号通路的调控作用,探讨其对DN大鼠的作用及产生该作用可能的机制。方法:采用一次性大剂量(60 mg·kg-1)腹腔注射链脲佐菌素(STZ)诱导建立DN大鼠模型。将DN大鼠随机分为正常组、模型组、厄贝沙坦组及当归-红芪超滤膜提取物低、中、高剂量组,之后开始灌胃给药,每天1次,8周后测空腹血糖(FBG),尿素氮(BUN),血肌酐(SCr),24 h尿蛋白;光镜观察肾组织结构变化;酶联免疫吸附测定(ELISA)检测大鼠TGF-β_1,Smad2/3含量;免疫组化法检测肾组织TGF-β_1,Smad2/3蛋白的表达;实时荧光定量聚合酶链式反应(Real-time PCR)法检测肾组织TGF-β_1,Smad2/3的mRNA表达。结果:与正常组比较,模型组大鼠FBG,BUN,SCr,24 h尿蛋白水平明显升高,ELISA检测大鼠TGF-β_1,Smad2/3含量明显升高,肾组织病理结构异常较为明显,TGF-β_1,Smad2/3蛋白量表达明显升高(P<0.05,P<0.01);与模型组比较,当归-红芪超滤膜提取物各组均不同程度降低了DN大鼠FBG,BUN,SCr,24 h尿蛋白水平(P<0.05);ELISA检测大鼠TGF-β_1,Smad2/3含量降低(P<0.05),肾组织病理结构异常得到改善,TGF-β_1,Smad2/3蛋白量表达减少(P<0.05,P<0.01),TGF-β_1,Smad2/3 mRNA表达减少(P<0.05,P<0.01)。结论:当归-红芪超滤膜提取物可改善DN大鼠肾功能病变,延缓DN慢性病理进展,其机制可能与其抑制TGF-β_1/Smad信号通路有关。
Objective： To study the regulatory effect of ultra-filtration extract from Angelice Sinensis Radix and Hedysari Radix （UFEASH） on transforming growth factor-β1（TGF-β1） and Smad signaling pathway of kidney tissue of rat with diabetic nephropathy （DN） , and explore its possible mechanism. Method ： The rat model with DN was induced by one-time high-dose （60 mg·kg^-1） intraperitoneal injection with streptozotocin （STZ）. All experimental rats were divided into blank group, model group, irbesartan group and low, median, high-dose UFEASH groups, and then intragastrieally administrated once a day. Fasting blood glucose, urea nitrogen （BUN） ,serum creatinine （SCr） , 24 h urinary protein were detected after 8 weeks. The changes in renal tissue structure were observed under light microscope. TGF-β1 and smad2/3 content of the rats were tested by ELISA. The expression of TGF-β1, Smad2/3 protein and mRNA expressions in renal tissues were detected by immunohistochemistry and Real-time PCR, respectively. Result： Compared with the normal group, the model group showed significant increases in FBG, BUN, SCr and 24 h urinary protein; ELISA showed notable increases in TGF-/31 and Smad2/3 content, remarkable renal pathological structural abnormality and notable increase in TGF- 131 and Smad2/3 protein expressions （P 〈 0.05, P 〈 0.01 ）. Compared with the model group, UFEASH groups significantly reduced the level of FBG, BUN, SCr and 24 h urine protein of rats with DN （P 〈 0.05 ）. The levels of TGF-β1 and Smad2/3 mRNA expressions were also decreased （P 〈 0.05 ）. The abnormal structure of renal tissue was alleviated. The expression of TGF-β1 and Smad2/3 were reduced （P 〈 0.05, P 〈 0.01 ）. Conclusion： UFEASH can improve renal function in rats with DN and delay the chronic pathological progress of DN, which may be related to the inhibition of TGF-β1/Smad signaling pathway.