文献类型：期刊文献

中文题名：清瘟解毒分散片质量标准研究

英文题名：Quality Standard of Qingwen Jiedu Dispersible Tablets

作者：王晓莉[1];刘成松[1]

第一作者：王晓莉

机构：[1]甘肃中医学院附属医院

第一机构：甘肃中医药大学第二附属医院

年份：2012

卷号：19

期号：1

起止页码：60

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：清瘟解毒分散片;黄芩苷;薄层色谱法;高效液相色谱法;质量标准

外文关键词：Qingwen Jiedu Dispersible Tablets; baicalin; TLC; HPLC; quality standard

摘要：目的建立清瘟解毒分散片的质量标准。方法采用薄层色谱法对处方中的玄参、牛蒡子、葛根进行定性鉴别,采用高效液相色谱法测定黄芩中黄芩苷的含量。色谱柱填充剂:十八烷基硅烷键合硅胶;流动相:甲醇-水-磷酸(47∶53∶0.2);检测波长:280 nm;流速:1.0 mL/min;柱温:25℃。结果定性鉴别专属性强,黄芩苷进样量在0.104～0.520μg范围内与峰面积积分值呈良好的线性关系(r=0.999 8),平均加样回收率为98.72%,RSD=1.31%(n=5)。结论所建立的标准可用于清瘟解毒分散片的质量控制。
Objective To establish the quality standard of Qingwen Jiedu Dispersible Tablets. Methods Radix scrophulariae, Fructus arctill and Radix puerariae in the formulation were identified by TLC. The content of baicalin was determined by HPLC with C18 column and using a mobile phase of MeOH-water-phosphonic acid （47∶53∶0.2） under the flow rate of 1.0 mL/min. The column temperature was 25 ℃ and the detection wavelength was 280 nm. Results The quanitative identification was specific. The linear range of the baicalin was 0.104-0.520 ？g （r=0.999 8） and the average recovery rate was 98.72% （RSD=1.31%, n=5）. Conclusion The eatablished standard is suitable for the quality control of Qingwen Jiedu Dispersible Tablets.