文献类型：期刊文献

英文题名：Hydroxytyrosol Alleviated Hypoxia-Mediated PC12 Cell Damage through Activating PI3K/AKT/mTOR-HIF-1α Signaling

作者：Li, Xiaolin[1,2,3];Tian, Xiuyu[1,2,4];Liu, Tianlong[1,2];Li, Maoxing[1,2,3,4,5];Wang, Weigang[1,2,3];Wang, Peng[1,2,3];Guo, Ziliang[1,2,4]

第一作者：李小林;Li, Xiaolin

通信作者：Li, MX[1];Li, MX[2];Li, MX[3];Li, MX[4];Li, MX[5]

机构：[1]940th Hosp Joint Logist Support Force Chinese Peop, Dept Clin Pharm, Lanzhou 730050, Peoples R China;[2]Gansu Plateau Pharmaceut Technol Ctr, Lanzhou 730050, Peoples R China;[3]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[4]Lanzhou Univ, Coll Pharm, Lanzhou 730000, Peoples R China;[5]Northwest Minzu Univ, Inst Chem Technol, Lanzhou 730030, Peoples R China

第一机构：940th Hosp Joint Logist Support Force Chinese Peop, Dept Clin Pharm, Lanzhou 730050, Peoples R China

通信机构：[1]corresponding author), 940th Hosp Joint Logist Support Force Chinese Peop, Dept Clin Pharm, Lanzhou 730050, Peoples R China;[2]corresponding author), Gansu Plateau Pharmaceut Technol Ctr, Lanzhou 730050, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Peoples R China;[4]corresponding author), Lanzhou Univ, Coll Pharm, Lanzhou 730000, Peoples R China;[5]corresponding author), Northwest Minzu Univ, Inst Chem Technol, Lanzhou 730030, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2022

卷号：2022

外文期刊名：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000811048100002)】；

基金：This work was supported by the Project of the Logistics Research Project (CLB21J036), the Fundamental Research Funds for the Central Universities (31920200010), the National Natural Science Foundation of China (82003982), the Scientific Research Program of Health Commission in Gansu Province (GSWSKY2020-41), the Natural Science Foundation of Gansu Province (20JR10R015), the Emergency Medical Research Project of Novel Coronavirus Pneumonia (COVID-19) (20yjky018), the Top-Notch Cultivation Project (2021yxky001), and the Youth Development Project of the 940th Hospital (2021yxky060).

语种：英文

摘要：Background. Hypoxia exerts pressure on cells and organisms, and this pressure can occur under both pathological and nonpathological conditions. There are many reports confirmed that hydroxytyrosol has good in vitro antioxidant activity, while the research about hydroxytyrosol on hypoxia-mediated cell damage is still unclear. Purpose. The aim of this study was to investigate the effect and mechanism of hydroxytyrosol on hypoxia-mediated cell damage. Methods. We studied the effects of hydroxytyrosol on the content of reactive oxygen species, the change of antioxidant enzymes activity of SOD, CAT, and GSH-Px and the content of oxidation products MDA and GSH, and the changes of cell membrane potential and effect on PI3K/AKT/mTOR-HIF-1 alpha signaling pathway under hypoxia-mediated PC12 cell. Results. PC12 cell treated with hydroxytyrosol abated the cell apoptosis and alleviated the oxidative stress through scavenging of reactive oxygen species, improving the enzyme activity and changing the content of oxidation products and alleviating mitochondria damage. Western blotting confirmed that the mechanism maybe related to the PI3K/AKT/mTOR-HIF-1 alpha signaling pathway. The inhibition experiment confirmed that hydroxytyrosol takes part in the expression of protein PI3K and p-mTOR. Conclusion. Hydroxytyrosol reduced the oxidative stress and resisted the inhibition of PI3K/AKT/mTOR-HIF-1 alpha signaling pathway caused by hypoxia, improved cell apoptosis, and ameliorated the antihypoxia ability of PC12 cells under hypoxia.