文献类型：期刊文献

中文题名：IL-1、IL-6、TNF-α及IFN-γ在脾肾阳虚型溃疡性结肠炎模型大鼠血清及组织中的表达

英文题名：Expression and significance of IL-1,IL-6,TNF-α and IFN-γ in serum and colon tissue in the rat models of ulcerative colitis with spleen and kidney yang deficiency

作者：殷银霞[1,2];许雅清[1];李海龙[1,3];邱家权[1];程小丽[1];明海霞[1];梁永林[1];吴玉泓[1]

第一作者：殷银霞

机构：[1]甘肃中医学院,兰州730000;[2]甘肃中医学院附属医院,兰州730020;[3]甘肃省中药新产品创制工程实验室,兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：23

期号：2

起止页码：139

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家自然科学基金项目(81260520)

语种：中文

中文关键词：溃疡性结肠炎;脾肾阳虚;白细胞介素;肿瘤坏死因子-α;干扰素-γ;大鼠

外文关键词：Ulcerative colitis;Yang deficiency of spleen and kidney;Interleukin;Tumor necrosis factor alpha;Interferon gamma;Enzyme linked immunosorbent assay;Rat

摘要：目的通过对脾肾阳虚型溃疡性结肠炎(UC)模型大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ表达水平的检测,探讨它们在UC发生发展过程中的作用。方法采用灌服大黄水煎液+肌肉注射氢化可的松并结合TNBS(2,4,6-三硝基苯磺酸)+乙醇灌肠建立脾肾阳虚型UC动物模型。将60只大鼠随机分为空白组、脾肾阳虚型UC模型7、14d及21d组,采用酶联免疫法检测各组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ的含量。结果与空白组比较,脾肾阳虚型UC模型组大鼠血清及结肠组织中IL-1、IL-6、TNF-α及IFN-γ含量明显升高(P<0.05);尤以模型21d组最为明显。结论促炎性细胞因子IL-1、IL-6、TNF-α及IFN-γ在脾肾阳虚型UC发病过程中起重要作用。
Objective To detect the levels of IL-1, IL-6,TNF-αand IFN-γin serum and colon tissue of rat mod-els of ulcerative colitis with spleen and kidney Yang deficiency, and to explore their roles in the pathogenesis of ulcerative colitis （ UC） .Methods The rat model of ulcerative colitis with Yang deficiency of spleen and kidney was induced by perfusion of rhubarb decoction plus intramuscular injection of hydrocortisone and combined with TNBS （2,4,6-trinitro-benzenesulfonic acid） and ethanol enema.Sixty SPF wistar rats （ body weight 180 ±10 g, male：female=1：1） were ran-domly divided into blank control group, UC model with spleen kidney Yang deficiency for 7 days, 14 days and 21 days groups, respectively.The levels of IL-1, IL-6, TNF-αand IFN-γin serum and colon tissue were detected by ELISA.Re-sults Compared with the blank group, the levels of IL-1, IL-6, TNF-αand IFN-γin serum and colon tissue of rat UC model group with spleen kidney Yang deficiency were greatly increased （P〈0.05）, especially evidently increased in the model group at 21 days.Conclusions The pro-inflammatory cytokines IL-1, IL-6, TNF-αand IFN-γplay an important role in the pathogenesis of ulcerative colitis with syndrome of spleen and kidney Yang deficiency.