文献类型：期刊文献

英文题名：Banxia Xiexin Decoction inhibits chemoresistance in gastric cancer by regulating BMSC-derived exosome-mediated G3BP1-YWHAZ protein interaction

作者：Yang, Fan[1,2];Liu, Senyu[3];Zhu, Zhongbo[1];Shi, Lijuan[1];Wang, Qingmiao[1];Niu, Kun[2];Liu, Xiping[1,4]

第一作者：杨帆;Yang, Fan

通信作者：Niu, K[1];Liu, XP[2]

机构：[1]Gansu Univ Chinese Med, Gansu Engn Lab New Prod Tradit Chinese Med, Gansu Key Lab TCM Excavat & Innovat Transformat, Lanzhou 730000, Gansu, Peoples R China;[2]Hainan Med Univ, Coll Tradit Chinese Med, Haikou 571199, Hainan, Peoples R China;[3]Hainan Med Univ, Affiliated Hosp 1, Haikou 571199, Hainan, Peoples R China;[4]Gansu Univ Chinese Med, 35 Ding Xi East Rd, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Hainan Med Univ, Coll Tradit Chinese Med, Haikou 571199, Hainan, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, 35 Ding Xi East Rd, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2026

卷号：18

期号：3

起止页码：2522

外文期刊名：AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001741282200003)】；

基金：This study was supported by the National Natural Science Foundation of China (No. 82260895) .

语种：英文

外文关键词：Banxia Xiexin Decoction; gastric cancer; exosomes; chemoresistance; G3BP1; YWHAZ

摘要：Objective: To investigate whether Banxia Xiexin Decoction (BXD) reverses bone marrow mesenchymal stem cell (BMSC)-derived exosome-induced oxaliplatin resistance in gastric cancer (GC) and to elucidate the underlying mechanism. Methods: Oxaliplatin-resistant HGC-27 cells and human BMSCs were cultured in vitro. Exosomes were isolated and characterized by transmission electron microscopy and marker analysis. Cell viability was assessed and G3BP1-YWHAZ interaction were examined by flow cytometry, immunofluorescence, Western blotting, and coimmunoprecipitation. A nude mouse xenograft model was used to evaluate in vivo effects. Results: BMSC-derived exosomes enhanced oxaliplatin resistance in HGC-27 cells, reduced apoptosis, upregulated MDR-related proteins, promoted SG formation, and strengthened G3BP1-YWHAZ interaction. BXD-containing serum reversed these effects by restoring apoptosis, increasing Bax and cleaved caspase expression, suppressing resistance-associated proteins and SG assembly, and disrupting G3BP1-YWHAZ binding. In vivo, BXD attenuated exosome-mediated chemoresistance, inhibited tumor growth, and enhanced oxaliplatin-induced apoptosis. Conclusion: BMSC-derived exosomes promote oxaliplatin resistance in GC through activation of the G3BP1-YWHAZ axis. BXD restores chemosensitivity by interfering with this exosome-mediated pathway, supporting its use as a potential adjuvant strategy to overcome chemotherapy resistance.