文献类型：期刊文献

中文题名：细胞膜色谱及质谱联用技术在中药活性成分筛选中的应用

英文题名：Application of Cell Membrane Chromatography Coupled with Mass Spectrometry in Screening of Active Ingredients of Traditional Chinese Medicines

作者：王玉[1];杨雪[2];夏鹏飞[1,3];杨蕊菁[1];王玉霞[1];杨飞霞[1];樊秦[1];武晓玉[1];彭雪晶[1,3];赵磊[1,3,4]

第一作者：王玉

机构：[1]甘肃中医药大学药学院,兰州730000;[2]北京中医药大学药学院,北京102488;[3]甘肃中医药大学甘肃省高校中藏药化学与质量研究省级重点实验室,兰州730000;[4]甘肃省道地药材质量标准化技术研究与推广工程实验室,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2020

卷号：26

期号：8

起止页码：241

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(81660577);甘肃省中医药管理局科研项目(G2KLP-2014-7);甘肃省中医药管理局科研项目(G2K-2014-77);甘肃省道地药材质量标准化技术研究与推广工程实验室开放基金项目(ddyc-2016-04)。

语种：中文

中文关键词：细胞膜色谱;质谱;联用;中药;活性成分;筛选

外文关键词：cell membrane chromatography;mass spectrometry;combination;traditional Chinese medicines;active compounds;screening

摘要：由于中药作用的整体性、成分和作用机制的复杂性,化学成分(群)作为中药及其复方制剂发挥治疗作用的物质基础,因此,采用多种方法分离分析中药及其复方中的化学成分一直是中药治病防病研究中一个有待解决的关键重要问题。传统方法存在操作繁琐、耗时耗事、浪费溶剂和样品的不可逆吸附等缺陷,并且分离过程中无法给出药理参数的基本信息,因而无法对活性成分进行高效准确定位。近年来从细胞层次研究药物成分与受体的结合为中药活性成分的筛选研究提供了一种新的途径。目前研究二者相互作用的方法之一-细胞膜色谱法(CMC),将现有色谱及质谱技术、细胞生物学与受体药理学结合起来,能够正确反映活性部位、活性成分与细胞膜及膜受体的相互作用,对复杂中药体系中有效部位筛选、活性成分的分离和高通量筛选具有独特优势。该文综述了CMC的原理、特点、中药活性成分筛选领域中的细胞膜模型及其与气相色谱-质谱(GC-MS)及液相色谱-质谱(LC-MS)联用的研究现状,并对其今后研究方法和未来的开发前景进行了探讨,为CMC在中医药领域的深入研究和开发利用提供理论依据和实践指导。
The chemical components(groups)contained in traditional Chinese medicine(TCM)and its compound preparations are the material basis for its curative effect,because of the integrity of the action of TCM and the complexity of its compositions and mechanism.The separation and analysis of chemical constituents in TCM and its compound prescriptions by various methods is always a key problem to be solved in the research of disease prevention and treatment of TCM.The binding of drug molecules to receptors at the cellular level was explored to provide a new idea for the screening of active components of TCM or its compound preparations.Traditional methods have some drawbacks,such as cumbersome operation,time-consuming,waste of solvents and irreversible adsorption of samples.The basic information of pharmacological parameters cannot be given in the separation process,and the active ingredients cannot be efficiently and accurately located.At present,cell membrane chromatography(CMC),one of the methods,is used to study the interaction between drug molecules and receptors,and can combine the existing chromatography and mass spectrometry technology,cell biology and receptor pharmacology,and correctly reflect the interaction between active parts,active components and cell membrane and membrane receptors,so it has unique advantages in screening effective parts,separation of active components and high-throughput screening from complex TCM system.The principles and characteristics of CMC,the cell membrane model in the field of active ingredient selection of TCM and its research status in combination with gas chromatography-mass spectrometry(GC-MS)and liquid chromatography-mass spectrometry(LC-MS)were reviewed,and its development prospects and future research methods were discussed,which provides theoretical basis and practical guidance for the research and utilization of CMC in the field of TCM.