文献类型：期刊文献

中文题名：金芪枳朮汤对GK糖尿病胃轻瘫大鼠胃窦组织平滑肌保护和修复作用

英文题名：Protecting and repairing effect of Jinqi Zhizhu Decoction on smooth muscle of gastric antrum in Goto-Kakizaki rat with diabetic gastroparesis

作者：万生芳[1];李雅琪[2];舒畅[1];张磊[1];王晓丽[1];魏昭晖[1];吴红彦[1]

第一作者：万生芳

机构：[1]甘肃中医药大学中医临床学院,兰州730000;[2]中国药科大学理学院,南京211198

第一机构：甘肃中医药大学中医临床学院

年份：2018

卷号：34

期号：19

起止页码：2327

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金资助项目(81560718);甘肃省普通本科高等学校基本科研业务费基金资助项目(2305136310);甘肃省中医药管理局科研课题基金资助项目(GZK-2017-3)

语种：中文

中文关键词：金芪枳朮汤;糖尿病胃轻瘫;胃泌素;胃动素;P物质;Rho;A/Rho相关激酶信号通路

外文关键词：Jinqi Zhizhu Decoction;diabetic gastroparesis;gastrin;motilin;substance P;RhoA/Rho - associated kinase signaling pathway

摘要：目的研究金芪枳朮汤对糖尿病胃轻瘫GK大鼠的胃动力影响与保护和修复胃窦平滑肌的作用及其机制。方法按照体重将9周龄雄性GK大鼠随机分为正常组(12只)和造模组(60只)。给予高糖高脂饲料不规则喂养8周制备糖尿病胃轻瘫模型。将成模大鼠随机分为5组:模型组、对照组(莫沙必利3.5μg·g^(-1)·d^(-1))和低、中、高3个剂量实验组(金芪枳朮汤6.9,13.8,27.6mg·g^(-1)·d^(-1)),每组12只,均连续灌胃给药12周。于干预前和干预后第12周末,检测大鼠血浆胃动素(MTL)、血清胃泌素(GAS)与P物质(SP)含量,以免疫印迹法检测RhoA与MYPT1的蛋白定量表达。结果用药12周后,模型组、对照组和低、中、高3个剂量实验组的GAS(ng·L^(-1))分别是48.83±2.31,83.39±3.10,73.03±3.92,79.57±3.46,92.33±3.99;这5组的MTL(pg·mL^(-1))分别是352.10±36.77,556.53±28.28,457.04±32.52,522.68±37.44,511.08±22.35;这5组的SP(ng·L^(-1))分别是119.94±9.95,288.97±8.18,242.53±10.97,254.59±11.13,270.35±8.81,对照组和低、中、高3个剂量实验组与模型组比较,差异均有统计学意义(P<0.05或P<0.01)。这5组的RhoA蛋白相对表达量分别是0.45±0.09,1.15±0.14,0.49±0.09,0.75±0.17,1.00±0.22;这5组的MYPT1蛋白相对表达量分别是3.48±0.49,5.01±0.40,3.12±0.20,4.00±0.25,4.86±0.29;对照组和高剂量实验组与模型组比较,差异有统计学意义(均P<0.01)。结论金芪枳朮汤能够提高胃激素含量,改善和修复平滑肌细胞的损伤,从而提高胃动力,改善糖尿病胃轻瘫症状。
Objective To explore the protective and repaired effect and its mechanism of Jinqi Zhizhu Decoction（ JQZZD） on smooth muscle of gastric antrum in Goto-Kakizaki（ GK） rat with diabetic gastroparesis（ DGP）. Methods Male GK rats（ 9-week-old） were randomly divided into normal group（ 12 rats） and building models（ 60 rats）,building models were induced to be DGP model by irregular feeding with high sugar and high fat diet for 8 weeks. The model rats were randomly divided into 5 groups,each group had 12 rats： model group,control group（ mosapride 3. 5 μg· g^-1· d^-1）,and low,middle,high-dose experimental groups（ JQZZD 6. 9,13. 8,27. 6 mg·g^-1·d^-1）. All rats except normal and model group were given intragastric administrationfor consective 12 weeks. Before and after intervention 12 weeks the concentrations of plasma motilin（ MTL）,serum gastrin（ GAS）,substance P（ SP） were detected by enzyme linked immunosorbent assay; the expressions of protein in RhoA and MYPT1 were detected by Western blot. Results The concentrations of GAS（ ng·L^-1） in model group,control group,and low,middle,high-doses experimental groups were 48. 83 ± 2. 31,83. 39 ± 3. 10,73. 03 ± 3. 92,79. 57 ± 3. 46,92. 33 ± 3. 99; the MTL（ pg · mL^-1） in the 5 groups were 352. 10 ± 36. 77,556. 53 ± 28. 28,457. 04 ± 32. 52,522. 68 ± 37. 44,511. 08 ± 22. 35; the SP（ ng · L^-1） in the 5 groups were 119. 94 ± 9. 95,288. 97 ± 8. 18,242. 53 ± 10. 97,254. 59 ± 11. 13,270. 35 ± 8. 81; comparing between control group and three doses experimental groups with model group,the differences were significant（ P〈0. 05 or P〈0. 01）. Relative amounts of protein expression of RhoA in the 5 groups were 0. 45 ± 0. 09,1. 15 ± 0. 14,0. 49 ± 0. 09,0. 75 ± 0. 17,1. 00 ± 0. 22; the MYPT1 in the 5 groups were 3. 48 ± 0. 49,5. 01 ± 0. 40,3. 12 ± 0. 20,4. 00 ± 0. 25,4. 86 ± 0. 29; comparing between control group and high dose experimental group with model group,the differences were significantly（ all P 0. 01）.Conclusion JQZZD may increase the concentration of gastric hormone and improve and repair damage of gastric antral muscle tissue,therefore improving gastric motility and symptoms of DGP.