文献类型：期刊文献

英文题名：Evaluation of an efficient and selective adsorbent based on multi-walled carbon nanotubes coated silica microspheres for detecting nucleobases and nucleosides in human urine

作者：Sun, Xiaoming[1,2];Tang, Yanan[1,2,3];Di, Duolong[1,2];Guo, Mei[3];Zhao, Lei[3]

第一作者：Sun, Xiaoming

通信作者：Di, DL[1]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[3]Gansu Coll Tradit Chinese Med, Dept Pharm, Coll Gansu Prov, Key Lab Chem & Qual Tradit Chinese Med, Lanzhou, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, 18 Tianshui Middle Rd, Lanzhou 730000, Peoples R China.

年份：2015

卷号：39

期号：7

起止页码：5513

外文期刊名：NEW JOURNAL OF CHEMISTRY

收录：;Scopus(收录号：2-s2.0-84933565449);WOS:【SCI-EXPANDED(收录号:WOS:000357106400062)】；

基金：This research was financially supported by the National Natural Science Foundation of China (NSFC No. 21205126) and Important Technology Plan Projects of Lanzhou City (2012-2-66). Thanks are due to Group 501 in Lanzhou Institute of Chemical Physics, ACS for assistance with the experiments.

语种：英文

摘要：Multi-walled carbon nanotubes (MWCNTs) can be used in analytical chemistry for separation and purification and offer the opportunity to determine low concentration compounds in complex systems. Novel multi-walled carbon nanotube coated silica microspheres (MWCNTs/SiO2) were synthesized by the covalent bonding of amino bonds. The characteristics results obtained from emission scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and Brunauer-Emmett-Teller (BET) surface area analysis showed that MWCNTs/SiO2 was successfully prepared. By evaluating the static adsorption capacities of ten nucleobases and nucleosides, the adsorption capacity of the microspheres for adenine (A), guanine (G), uric acid (UA) and xanthosine (X) was found to be significantly stronger than for others. Moreover, the adsorption capacity depends on the pH value, salinity and contact time. It was shown that pi-pi conjugation and hydrogen bonding interactions were the two main driving forces for the adsorption of target compounds. Adsorption kinetics and adsorption isotherms showed that the adsorption process was a chemical and multilayer adsorption. They could be determined within the test ranges with a good correlation coefficient (r > 0.997). The limits of detection (LOD) for A, G, UA and X were 1.22, 2.02, 0.32 and 2.28 ng mL(-1), respectively. The intra-and inter-day relative standard deviations (RSDs) were no more than 6.5%. This procedure therefore afforded a convenient, sensitive and accurate method with a high extraction efficiency for the determination of A, G, UA and X in human urine.