文献类型：期刊文献

中文题名：热补针法调控Sirt1/FoxO1通路对类风湿性关节炎寒证大鼠氧化应激损伤的影响

英文题名：Heat-reinforcing needling inhibits oxidative stress injury via modulating the Sirt1/FoxO1 pathway in rheumatoid arthritis rats with cold syndrome

作者：吴永吉[1];张宏涛[2];张星华[2];任超展[2];颉旺军[1];张广军[3]

第一作者：吴永吉

机构：[1]甘肃中医药大学针灸推拿学院,兰州730000;[2]甘肃省中医院针灸一科,兰州730050;[3]兰州市中医医院风湿骨病科,兰州730050

第一机构：甘肃中医药大学针灸推拿学院

年份：2025

卷号：50

期号：7

起止页码：808

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：;北大核心:【北大核心2023】；

基金：甘肃省自然科学基金项目(No.22JR5RA637)。

语种：中文

中文关键词：类风湿关节炎;热补针法;氧化应激;沉默信息调节因子1(Sirt1)/叉头盒转录因子O1(FoxO1)信号通路

外文关键词：Rheumatoid arthritis;Heat-reinforcing needling;Oxidative stress;Sirt1/FoxO1 pathway

摘要：目的:观察热补针法针刺“足三里”对类风湿性关节炎(RA)寒证大鼠炎性反应及氧化应激的影响,探讨热补针法通过调控沉默信息调节因子1(Sirt1)/叉头盒转录因子O1(FoxO1)信号通路改善RA寒证的机制。方法:雄性SD大鼠按随机数字表法随机分为空白组、模型组、热补针法组、白藜芦醇组,每组12只。采用牛Ⅱ型胶原蛋白与完全弗氏佐剂联合寒、湿环境因素复合造模法制备RA寒证大鼠模型。造模成功后,白藜芦醇组腹腔注射Sirt1激动剂白藜芦醇(10 mg/kg),热补针法组于“足三里”行热补针法针刺,留针30 min。每组均干预1次/d,连续治疗14 d。HE染色法观察大鼠膝关节滑膜组织形态学;分别以WST-1法、比色法、探针法、TBA法检测各组大鼠血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及活性氧(ROS)、丙二醛(MDA)的含量;Western blot法检测各组大鼠膝关节滑膜组织Sirt1、FoxO1蛋白的表达水平;实时荧光定量PCR法检测各组大鼠膝关节滑膜组织Sirt1、FoxO1 mRNA的表达。结果:与空白组相比,模型组大鼠膝关节滑膜组织周围纤维组织增生、炎性细胞浸润,Sirt1 mRNA及蛋白,SOD、GSH-Px活性显著降低(P<0.01),FoxO1 mRNA及蛋白,ROS、MDA含量显著升高(P<0.01);与模型组相比,热补针法组和白藜芦醇组大鼠膝关节滑膜组织中Sirt1 mRNA及蛋白,SOD、GSH-Px活性显著升高(P<0.01,P<0.05),FoxO1 mRNA及蛋白,ROS、MDA含量显著降低(P<0.01),且膝关节滑膜组织周围纤维组织增生、炎性细胞浸润明显减轻。结论:热补针法能够通过调控Sirt1/FoxO1通路,减轻RA氧化应激损伤,并可能通过抑制氧化应激进而减轻炎性反应,从而达到缓解RA的治疗作用。
Objective To observe the effects of heat-reinforcing needling on inflammation and oxidative stress in rheumatoid arthritis(RA)model rats with cold syndrome,so as to analyze the mechanism of heat-reinforcing needling in improving RA.Methods Male SD rats were randomly divided into blank group,model group,heatreinforcing needling group and resveratrol group according to random number table method,with 12 rats in each group.The rat model of RA with cold syndrome was established by subcutaneous injection of bovine type II collagen and complete Freund adjuvant combined with the stimulation of cold and wet environment.After successful modeling,rats of the resveratrol group was intraperitoneally injected with resveratrol(the agonist of Sirt1,10 mg/kg),and rats of the heat-reinforcing needling group received heat-reinforcing needling at“Zusanli”(ST36)for 30 min every day.After 14 d of continuous intervention,the synovial morphology of knee joint was observed by HE staining.The activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),contents of reactive oxygen species(ROS)and malondialdehyde(MDA)in serum were detected by WST-1 method,colorimetric method probe method,and TBA method,respectively.The mRNA and protein expression levels of silencing information regulatory factor 1(Sirt1)and(FoxO1)in the synovial tissue of knee joint were detected by Western blot and qPCR,respectively.Results Compared with blank group,the fibroplasia,inflammatory cell infiltration,the relative expressions of Sirt1 mRNA and protein in synovial of knee joint,serum SOD and GSH-Px activity in model group were significantly decreased(P<0.01),while the relative expressions of FoxO1 mRNA and protein,the contents of ROS and MDA were significantly increased(P<0.01).After interventions,the decreased Sirt1 mRNA and protein,decreased SOD and GSH-Px activities,and the increased FoxO1 mRNA and protein,increased contents of ROS and MDA were reversed(P<0.01,P<0.05)in both of the heat-reinforcing needling and resveratrol groups.At the same time,the fibrous tissue proliferation and inflammatory cell infiltration around the synovial tissue of knee joint induced by RA were significantly reduced in both of the intervention groups.Conclusion Heat-reinforcing needling can inhibit oxidative stress injury and inflammation response in RA rats with cold syndrome,which may be related to the regulation of Sirt1/FoxO1 pathway.