文献类型：期刊文献

英文题名：Neurone-satellite glial cell interactions in dorsal root ganglia drive peripheral sensitisation in a mouse burn pain model

作者：Zhang, Run[1,2,3,4];Zhang, Nan[1,2,3];Chen, Dan[1,2,3];Hu, Xuanran[1,2,3];Zhang, Mengna[1,2,3];Yao, Minhua[5];Zhang, Qinqin[1,2,3];Wu, Shuyuan[1,2,3];Zhang, Xiaodi[1,2,3];He, Yongtao[1,2,3];Gao, Feiyun[1,2,3];Xu, Biao[1,2,3];Fang, Quan[1,2,3]

第一作者：Zhang, Run;张蕊

通信作者：Xu, B[1];Fang, Q[1];Xu, B[2];Fang, Q[2];Xu, B[3];Fang, Q[3]

机构：[1]Lanzhou Univ, Coll Vet Med, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Gansu, Peoples R China;[2]Lanzhou Univ, Inst Physiol, Coll Vet Med, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Coll Vet Med, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Inst Physiol, Sch Basic Med, Lanzhou, Gansu, Peoples R China;[5]Lanzhou Univ, Inst Anat & Histol & Embryol, Sch Basic Med Sci, Neurosci, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Coll Vet Med, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Coll Vet Med, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Lanzhou Univ, Inst Physiol, Coll Vet Med, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China;[3]corresponding author), Lanzhou Univ, Coll Vet Med, State Key Lab Vet Etiol Biol, Lanzhou, Gansu, Peoples R China.

年份：2025

卷号：135

期号：2

起止页码：416

外文期刊名：BRITISH JOURNAL OF ANAESTHESIA

收录：;Scopus(收录号：2-s2.0-105005573587);WOS:【SCI-EXPANDED(收录号:WOS:001550838900013)】；

基金：National Natural Science Foundation of China (82301392 to BX) ; the Funds for the Major Science and Technology Project of Gansu Province (23ZDNA007 to QF) ; and the Natural Science Foundation of Gansu Province (24JRRA1031 to RZ, 22JR5RA545 to BX) .

语种：英文

外文关键词：burn pain; CGRP; chemokines; gap junction; neurone-SGC interaction

摘要：Background: Accumulating evidence suggests that glial mechanisms are pivotal in regulating chronic pain. Our previous findings revealed that the interactions between spinal microglia and astrocytes are crucial for burn-induced pain hypersensitivity. However, the mechanisms underlying burn-induced peripheral sensitisation remain incompletely understood. Methods: Sensory neurone-satellite glial cell (SGC) interactions within peripheral dorsal root ganglia were investigated using in vitro and in vivo experiments. Behavioural tests were conducted to evaluate the therapeutic potential of targeting peripheral sensitisation mechanisms for burn pain management. Results: Burn injury upregulated calcitonin gene-related peptide (CGRP) expression in sensory neurones (1.5-fold; P1/40.013) through transient receptor potential vanilloid 1 (TRPV1) channels. Pharmacological blockade of the TRPV1/CGRP signalling pathway effectively attenuated burn-induced mechanical allodynia and thermal hyperalgesia. Additionally, neurone-derived CGRP triggered SGC activation (from 6.8% pre-injury to 41.6% at day 5 post-injury), concomitant with enhanced gap junction-mediated SGC coupling (from 16.7% pre-injury to 40.5% at day 5 post-injury). Furthermore, chemokine expression (particularly CXCL1) in SGCs was elevated after burn injury, which potentiated sensory neurone excitability and exacerbated pain hypersensitivity. Blocking SGC coupling exerted potent analgesic effects in this burn pain model. Conclusions: A novel neurone-SGC interaction mechanism drives burn-induced peripheral sensitisation, providing translational implications for burn pain therapeutics.