文献类型：期刊文献

英文题名：Porous graphene-black phosphorus nanocomposite modified electrode for detection of leptin

作者：Cai, Jinying[1];Gou, Xiaodan[2];Sun, Bolu[1];Li, Wuyan[3];Li, Dai[1];Liu, Jinglong[1];Hu, Fangdi[1];Li, Yingdong[4]

第一作者：Cai, Jinying

通信作者：Hu, FD[1];Li, YD[2]

机构：[1]Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210046, Jiangsu, Peoples R China;[3]Temple Univ, Sch Med, Ctr Inflammat Translat & Clin Lung Res, Philadelphia, PA USA;[4]Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Affiliated Hosp, Lanzhou 730000, Gansu, Peoples R China.|[10735b845793de6ae2b30]甘肃中医药大学第二附属医院;[10735]甘肃中医药大学;

年份：2019

卷号：137

起止页码：88

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20192006912986);Scopus(收录号：2-s2.0-85065414459);WOS:【SCI-EXPANDED(收录号:WOS:000471359200011)】；

基金：The authors acknowledge the National Key Research and Development Program of China (2018YFC1706300-3), Special funds for Innovation and Guidance of Gansu (2017ZX-02), the Science Technology plan Project from Lanzhou Science and Technology Bureau (2016-2-80, 2017-4-119, 2017-RC115), the Key R&D Project of Gansu Provincial Science and Technology Department (17ZD2FA009), the National Traditional Chinese Medicine Standardization Project (ZYBZH-YGS-10), and the Key Project of the Fundamental Research Funds for the Central Universities (lzujbky-2018-k13, lzujbky-2017-205), and the project form Gansu Medical Products Administration.

语种：英文

外文关键词：Non-alcoholic fatty liver disease; Leptin; Electrochemical immunosensor; Porous graphene-black phosphorus; Label-free

摘要：Leptin is a vital biomarker of non-alcoholic fatty liver (NAFLD), and its evaluation of the concentration level in vivo is of great significance to NAFLD diagnosis. Therefore, it is pressing to develop a method for rapid and sensitive detection of leptin. This paper describes an environmentally friendly and label-free immunosensor based on porous graphene functionalized black phosphorus (PG-BP) composite to detect of leptin. The PG-BP was synthesized via strong coherent coupling between porous graphene (PG) surface plasmons and anisotropic black phosphorus (BP) localized surface plasmons, which made the electrochemical performance of PG and BP synergistic as well as increased the stability and conductive capability of BP material. The PG-BP modified electrodes was further prepared by gold nanoparticles, cysteamine, and glutaraldehyde in turn. Due to the cross-linking effect of glutaraldehyde, anti-leptin can be firmly fixed. These properties of the platform improved the conductive capability of the immunosensor and enhanced the load capacity of the proteins, thereby, the sensitivity of the immunosensor was significantly increased. Under the optimal conditions, the proposed immunosensor exhibited a wide linear range of 0.150-2500 pg/mL with a low detection limit of 0.036 pg/mL. The leptin immunosensor displayed excellent selectivity and anti-interference ability, which could be used for early screening and diagnosis of clinical NALFD.