文献类型：期刊文献

中文题名：基于抗氧化研究三七提取物对6-OHDA损伤PC12细胞的保护作用

英文题名：Protective Effect of Sanqi(三七)Extract on 6-OHDA-injured PC12 cells Based on Antioxidation Study

作者：姚娟[1];林佳[1];吴平安[1];靳晓杰[1]

第一作者：姚娟

机构：[1]甘肃中医药大学药学院,西北中藏药协同创新中心,甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）|甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2022

卷号：38

期号：2

起止页码：115

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金地区科学基金项目(编号:81960823);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金项目(编号:zzy-2016-05);甘肃中医药大学引进人才科研启动基金项目(编号:2016YJRC-04);甘肃省自然科学基金项目(编号:21JR1RA270);甘肃省教育厅高等学校产业支撑计划项目(编号:2020C-15)

语种：中文

中文关键词：三七;抗氧化;自由基;氧化损伤;凋亡

外文关键词：Sanqi(三七);Antioxidant;Free radicals;Oxidative damage;Apoptosis

摘要：目的:研究三七提取物的体外自由基清除活性及对6-羟基多巴胺(6-OHDA)损伤PC12细胞的保护作用。方法:使用50%乙醇超声提取三七有效成分,检测三七提取物对ABTS及DPPH自由基的清除活性;采用MTT法评价提取物对PC12细胞的活性,测定细胞培养基中乳酸脱氢酶(LDH)的含量,评价三七提取物对6-OHDA损伤PC12细胞的保护作用,2,7-二氯二氢荧光素二乙酸酯(DCFH-DA)染色测定三七提取物对6-OHDA损伤PC12细胞对活性氧的影响,检测半胱氨酸蛋白酶3(Caspase 3)的蛋白表达及活性观察对Caspase 3相关凋亡的影响,通过检测细胞中总谷胱甘肽(GSH)、超氧化物歧化酶(SOD)的活性及总抗氧化能力(T-AOC)评价内源性的抗氧化能力。结果:与空白对照组相比,三七提取物0.3 mg/mL~1.0 mg/mL各浓度均能够显著清除ABTS及DPPH自由基;与模型对照组相比,三七提取物2、5、10 mg/L处理PC12细胞后能显著提高6-OHDA损伤环境下PC12细胞的活性、清除细胞中的部分活性氧、抑制Caspase 3的蛋白表达及活性,提高6-OHDA损伤环境下PC12细胞中总GSH、T-AOC的含量及SOD活力。结论:三七提取物对体外自由基具有清除作用;对6-OHDA损伤环境下的PC12细胞具有一定的保护作用,能抑制PC12细胞中与Caspase 3相关的凋亡,其机制可能与参与上调生物系统内源性的抗氧化作用相关。
Objective:To investigate the in vitro free radicals-scavenging activity of Sanqi(三七)extract and the protection on 6-hydroxydopamine(6-OHDA)-damaged PC12 cells.Methods:Effective components of Sanqi were extracted by sonication with 50%ethanol,and the ABTS-and DPPH-scavenging activity of Sanqi extract in vitro was determined.The activity of PC12 cells was detected by methyl thiazolyl tetrazolium(MTT)assay.The lactate dehydrogenase(LDH)content in cell culture medium was detected,and the protective effect of Sanqi extract on 6-OHDA-damaged PC12 cells were measured.The 2,7-dichlorodihydrofluorescein diacetate(DCFH-DA)staining was used to detect the influence of Sanqi extract on the reactive oxygen species(ROC)in 6-OHDA-damaged PC12 cells.The expression and activity of Caspase 3 were detected,and the effect of Caspase 3-related apoptosis was observed.Then the content of total glutathione(GSH)and superoxide dismutase(SOD),and total antioxidant capacity(T-AOC)were detected to evaluate the endogenous antioxidant effect.Results:Compared with normal control group,0.3 mg/mL~1.0 mg/mL Sanqi extract can directly scavenge ABTS and DPPH.Compared with model group,2,5,and 10 mg/L Sanqi extract significantly improved the activity of 6-OHDA-damaged PC12 cells,reduced ROS accumulation in cells,inhibited the activity and expression of Caspase-3,and raised the content of GSH and T-AOC and SOD activity in 6-OHDA-damaged PC 12 cells.Conclusion:Sanqi extract can scavenge free radicals,protect the 6-OHDA-damaged PC-12 cells,and inhibit the Caspase 3-related apoptosis,which may be related to the regulation of endogenous antioxidation.