文献类型：期刊文献

中文题名：归芪益元膏对重离子辐射旁效应损伤大鼠肺肾组织caspase-3及caspase-9表达的影响

英文题名：Influence of Guiqiyiyuan Ointment on the expression of caspase-3 and caspase-9 in rat lung tissues injured by heavy ion radiation-induced bystander effect

作者：张朝宁[1,2];李金田[1,2];刘永琦[2];蔺兴遥[2];孙少伯[2];李娟[1,2];李雪燕[2]

第一作者：张朝宁

机构：[1]兰州甘肃中医药大学中医临床学院,730000;[2]敦煌医学与转化省部共建教育部重点实验室

第一机构：甘肃中医药大学中医临床学院

年份：2018

卷号：43

期号：4

起止页码：278

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金(81460695)

语种：中文

中文关键词：辐射旁效应;重离子;归芪益元膏

外文关键词：radiation induced bystander effect; heavy ion; Guiqiyiyuan Ointment

摘要：目的探讨归芪益元膏对重离子(^(12)C^(6+))辐射旁效应损伤大鼠肺肾组织中caspase-3及caspase-9表达的影响。方法 42只雄性Wistar大鼠随机分为7组,即正常对照组(NC组)、单纯辐射组(RO组)、中药组(CM组),后两组按照射后处死时间分为6、12、24h亚组,每组6只。CM组预先用归芪益元膏灌胃2周,NC组和RO组用等量生理盐水灌胃;2周后RO组和CM组大鼠右肺给予2Gy ^(12)C^(6+)离子束单次照射,NC组不照射。于照射后6、12、24h处死各组大鼠,RTqPCR及免疫组化检测大鼠右肺、左肺、左肾组织中caspase-3、caspase-9 mRNA及蛋白的表达。结果与NC组比较,RO组大鼠右肺、左肺、左肾caspase-3、caspase-9 mRNA表达在照射后6、12、24h均明显升高,而蛋白表达仅在照射后24h明显升高,差异有统计学意义(P<0.01)。CM组大鼠右肺、左肺、左肾caspase-3、caspase-9 mRNA及蛋白表达较同时间点的RO组明显下调,差异有统计学意义(P<0.01)。结论归芪益元膏可抑制大鼠肺肾组织caspase-3、caspase-9表达的上调,从而减轻重离子辐射诱导的体内旁效应损伤。
Objective To explore the influence of Guiqiyiyuan Ointment on the expressions of caspase-3 and caspase-9 in the lung and kidney of the rats damaged by heavy ion（^12C^6＋） radiation-induced bystander effect. Methods The Wistar male rats were equally and randomly divided into seven groups, normal control group（NCG）, radiation alone group（RAG） and Chinese medicine group（CMG）, with the latter two groups being redivided into 6, 12 and 24 h groups according to the executing time. The Chinese medicine groups were given Guiqiyiyuan Ointment by gavage for two weeks in advance. The normal control group and the radiation alone groups were given the equal normal saline. Afterwards, the right lung of the rats in the radiation alone groups and Chinese medicine groups were radiated by 2 Gy -（12）C-（6＋）ion once. The rats in normal control group were not radiated. All groups of rats were executed 6, 12, and 24 h after radiation. The protein and m RNA expressions of caspase-3 and caspase-9 in the right lung, left lung and left kidney were examined with immunohistochemistry and Q-PCR. Results Compared with the normal control group, the m RNA expressions of caspase-3 and caspase-9 in the right lung, left lung and left kidney in the radiation alone groups obviously increased 6 and 24 h after radiation. While the protein expressions of caspase-3 and caspase-9 in the radiation alone group obviously increased only 24 h after radiation（P〈0.01）. Compared with the radiation alone groups, the expressions of protein and m RNA of caspase-3 and caspase-9 were obviously down-regulated in the Chinese medicine groups（P〈0.01）. Conclusion By controlling the up-regulation of the expression of caspase-3 and caspase-9, Guiqiyiyuan Ointment can prevent the lung and kidney cell apoptosis and alleviate the damage caused by heavy ion radiation-induced bystander effect in vivo.