文献类型：期刊文献

英文题名：Holistic analysis of seven constituents from five medicinal herbs composing Wuhu powders in a single run by ultra performance liquid chromatography: application to quality control study

作者：Chen, Juan[1];Jin, Ling[2];Li, Ying-Dong[2];Shi, Yan-Ping[1]

第一作者：Chen, Juan

通信作者：Shi, YP[1]

机构：[1]Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China;[2]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Peoples R China

第一机构：Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China.

年份：2013

卷号：5

期号：24

起止页码：7058

外文期刊名：ANALYTICAL METHODS

收录：;EI(收录号：20134917046692);Scopus(收录号：2-s2.0-84888315829);WOS:【SCI-EXPANDED(收录号:WOS:000327506600026)】；

基金：This work has been supported by the National Key Technology Research and Development Program of China (no. 2011BAI05B02) and the National Natural Science Foundation of China (no. 21105106).

语种：英文

外文关键词：Linoleic acid - Liquid chromatography - Medicine - Quality assurance

摘要：The newly proposed strategy, namely, holistic analysis of multiple constituents from every constituent herb in a single run, was applied for the quality control of traditional Chinese medicine preparation Wuhu powder. Seven bioactive components, i.e., ferulic acid, hydroxysafflor yellow A, prim-O-glucosylcimifugin, 4 '-O-beta-D-glucosyl-5-O-methylvisamminol, imperatorin, isoimperatorin, and linoleic acid, from 5 medicinal herbs composing Wuhu powder were selected as evaluation markers, and then their quantitative information was obtained in a single run by an UPLC method coupled with photodiode array detection. Optimum separation was achieved on a C-18 column (50 x 2.1 mm i.d., 1.7 mu m particle) through a gradient delivery of a mixture of acetonitrile and 0.1% aqueous phosphoric acid at a flow rate of 0.3 mL min(-1) at 30 degrees C. The corresponding maximum absorption wavelengths were selected as the detection wavelengths for each analyte. For all of the analytes, a good linear relationship (r of >0.999) was obtained between peak area and concentration. The method was validated for repeatability, precision, stability, accuracy, and selectivity. The RSD values of retention time and peak area were 0-0.6% and 0.7-1.0% for intra-assay, and 0.1-1.0% and 1.6-1.8% for inter-assay precision. Analysis repeatability and stability (24 h) were all lower than 5.0%. The recovery of the method was 96.1-97.7% with a RSD of 2.4-4.0%. The validated method was applied to quality control of Wuhu powder from different production batches.