文献类型：期刊文献

中文题名：附子-干姜对慢性溃疡性结肠炎小鼠肠黏膜微循环障碍的改善作用

英文题名：Effect of Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma on intestinal mucosal microcirculation in mice with chronic ulcerative colitis

作者：翟勇聪[1];夏顺利[1];王雪[1];臧凯宏[1,2];韩涛[1,2]

第一作者：翟勇聪

机构：[1]甘肃中医药大学药学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2021

卷号：52

期号：7

起止页码：1987

中文期刊名：中草药

外文期刊名：Chinese Traditional and Herbal Drugs

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金地区基金资助项目(81860728)。

语种：中文

中文关键词：附子-干姜;慢性溃疡性结肠炎;肠黏膜;微循环;微血管密度

外文关键词：Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma;chronic ulcerative colitis;intestinal mucosa;microcirculation;microvessel density

摘要：目的研究附子-干姜对慢性溃疡性结肠炎(ulcerativecolitis,UC)小鼠肠黏膜微循环障碍的改善作用。方法建立慢性UC小鼠模型,给予附子-干姜水煎液进行干预,记录各组小鼠体质量、大便性状及便血情况,进行疾病活动指数(disease activityindex,DAI)评分;采用激光多普勒血流仪检测各组小鼠肠黏膜血流灌注量、血细胞移动速度和移动血细胞浓度;采用ELISA法检测各组小鼠血清中血小板活化因子(platelet activating factor,PAF)和组织因子(tissue factor,TF)水平;采用苏木素-伊红(HE)染色法观察各组小鼠结肠组织病理改变情况;采用免疫组化法检测各组小鼠肠黏膜毛细血管内皮细胞CD31表达并计算微血管密度(microvessel density,MVD)。结果与对照组比较,模型组小鼠DAI评分显著增加(P<0.01),肠黏膜血流灌注量、血细胞移动速度显著下降(P<0.01),肠黏膜移动血细胞浓度显著升高(P<0.01),血清PAF、TF水平显著升高(P<0.01),结肠组织CD31表达减少,肠黏膜微血管密度显著减少(P<0.01);与模型组比较,附子-干姜高剂量(6.06 g/kg)组小鼠DAI评分明显降低(P<0.01),肠黏膜血流灌注量、血细胞移动速度显著升高(P<0.01),肠黏膜移动血细胞浓度显著降低(P<0.01),机体凝血因子PAF、TF水平显著降低(P<0.01),肠黏膜MVD显著增加(P<0.01)。结论附子-干姜可通过改善肠黏膜微循环障碍从而治疗慢性UC。
Objective To study the effect of Fuzi(Aconiti Lateralis Radix Praeparaia)-Ganjiang(Zingiberis Rhizoma) on intestinal mucosal microcirculation disorder in mice with chronic ulcerative colitis(UC). Methods Mice model of chronic UC was established, and Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma decoction was given to intervene. Body weight, stool characteristics and blood in stool were recorded, disease activity index(DAI) score was performed. Laser Doppler Blood Flow Meter was used to detect intestinal mucosal blood perfusion, blood cell movement speed and mobile blood cell concentration. ELISA method was used to detect platelet activating factor(PAF) and tissue factor(TF) levels in serum. Pathological changes of colon tissue were observed by hematoxylin-eosin(HE) staining. Immunohistochemical method was used to detect the expression of CD31 in intestinal mucosal capillary endothelial cells of mice and the microvessel density(MVD) was calculated. Results Compared with control group, DAI score of mice in model group was significantly increased(P < 0.01), intestinal mucosal blood perfusion and blood cell moving speed were significantly decreased(P < 0.01), the concentration of moving blood cells in intestinal mucosa was significantly increased(P < 0.01), PAF and TF levels in serum were significantly increased(P < 0.01), CD31 expression in colon tissue was reduced, and intestinal mucosal microvessel density was significantly reduced(P < 0.01). Compared with model group, DAI score of mice in high-dose(6.06 g/kg) Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma group was significantly reduced(P < 0.01), intestinal mucosal blood perfusion and blood cell moving speed were significantly increased(P < 0.01), concentration of moving blood cells in intestinal mucosa was significantly reduced(P < 0.01), and coagulation factor PAF and TF levels in serum were significantly reduced(P < 0.01), MVD of intestinal mucosa was significantly increased(P < 0.01). Conclusion Aconiti Lateralis Radix Praeparaia-Zingiberis Rhizoma can treat chronic UC by improving intestinal mucosal microcirculation.