文献类型：期刊文献

中文题名：当归红芪超滤膜提取物通过调控NOX家族改善糖尿病肾病氧化应激的作用机制

英文题名：Mechanism of improving oxidative stress in diabetic kidney disease by regulating NOX family through ultrafiltration membrane extract of Angelica sinensis and Radix Hedysari

作者：郭倩[1];万生芳[1,2];邵晶[3];李荣科[1];魏昭晖[1];张磊[1]

第一作者：郭倩

机构：[1]甘肃中医药大学基础医学院,甘肃兰州730000;[2]甘肃中医药大学敦煌医学与转化教育部重点实验室,甘肃兰州730000;[3]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2025

卷号：41

期号：8

起止页码：1584

中文期刊名：中国药理学通报

外文期刊名：Chinese Pharmacological Bulletin

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金资助项目(No 82060914);甘肃中医药大学2022年度中医学一级学科“岐黄英才”导师专项基金(博导-6、硕导-12);敦煌医学与转化教育部重点实验室开放课题(No DHYX23-02);甘肃中医药大学研究生“创新创业基金”项目(No 2025CXCY-004)。

语种：中文

中文关键词：当归红芪超滤膜提取物;糖尿病肾病;血瘀证;氧化应激;NOX家族;作用机制

外文关键词：ultrafiltration membrane extract of Angelica sinensis and Radix Hedysari;diabetic kidney disease;blood stasis;oxidative stress;NOX family;mechanism of action

摘要：目的探讨当归红芪超滤膜提取物对糖尿病肾病(diabetic kidney disease,DKD)大鼠氧化应激的机制研究。方法将45只SD大鼠随机分为正常组(8只)、造模组(37只)。造模组高糖高脂饲料喂养4周后腹腔注射链脲佐菌素30 mg·kg^(-1)诱导建立糖尿病大鼠模型。3周后将24 h尿蛋白(24-hour urinary protein,24-hUP)≥30 mg大鼠按0.05 mg·kg^(-1)尾静脉注射10%大分子右旋糖苷3次,诱导成DKD血瘀证模型。检测大鼠随机血糖(glucose,GLU)、24-hUP、生化指标、病理染色等;免疫印迹检测肾组织烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate,NADPH)氧化酶(NADPH oxidase,NOX)1、NOX2、NOX3、NOX4、NOX5的蛋白表达。结果与正常组相比,模型组大鼠GLU、24-hUP、SCr、BUN、TG、TC明显升高,ALB2、HDL、LDL显著降低,NOX1、NOX2、NOX4、NOX5蛋白相对表达明显升高(P<0.01);与模型组相比,给药组大鼠6周、8周GLU、24-hUP、SCr、BUN、TG、TC均明显降低,ALB2、HDL、LDL明显升高,NOX1、NOX2、NOX4、NOX5蛋白相对表达明显降低(P<0.05)。结论当归红芪超滤膜提取物可有效改善DKD氧化应激及大鼠肾功能,其作用机制靶点可能与NOX家族有关。
Aim To investigate the mechanisms of the ultrafiltration membrane extract of Angelica sinensis and Radix Hedysari extracts on oxidative stress in rats with diabetic kidney disease(DKD).Methods Forty-five SD rats were randomly divided into a control group(n=8)a model group(n=37).Rats in the model group were fed a high-sugar,high-fat diet for four weeks,followed by intraperitoneal injection of streptozotocin at a dose of 30 mg·kg^(-1) to induce diabetes in the rats.Three weeks later,rats with 24-hour urinary protein(24-hUP)levels more than or equal to 30 mg were injected via the tail vein with 0.05 mg·kg^(-1) of 10%high molecular weight dextran for three times to induce a model of blood stasis in diabetic kidney disease(DKD).The rats were then evaluated for random blood glucose(GLU)levels,24-hUP,biochemical markers,histopathological staining,and the protein expression of nicotinamide adenine dinucleotide phosphate(NADPH)oxidase(NOX)1,NOX2,NOX3,NOX4,and NOX5 in renal tissues using immunoblot analysis.Results Compared to the control group,rats in the model group showed significantly increased GLU,24-hUP,SCr,BUN,TG,TC,bu markedly decreased ALB2,HDL,LDL levels,and the relative expression of NOX1,NOX2,NOX4,NOX5 proteins increased markedly(P<0.01);Comparison with the model group,rats in the treatment group exhibited significantly decreased GLU,24-hUP,SCr,BUN,TG,TC at 6 weeks and 8 weeks,but markedly increased ALB2,HDL,LDL levels,and the relative expression of NOX1,NOX2,NOX4,NOX5 proteins decreased significantly(P<0.05).Conclusions The ultrafiltration membrane extract of Angelica sinensis and Radix Hedysari can effectively ameliorate oxidative stress and renal function in DKD rats,which may be associated with targets within the NOX family.