文献类型：期刊文献

中文题名：芍药汤对湿热内蕴型溃疡性结肠炎大鼠TLR4,NF-κB p65和IL-6表达的调控作用

英文题名：Effect of Shaoyaotang on Expressions of TLR4,NF-κB p65 and IL-6 in Rats with Damp-heat Ulcerative Colitis

作者：徐敏[1];王凤仪[1];赵党生[1];蒲晓薇[1];张磊[1];张小元[1];李琳[1];李淑霞[1];李志平[1]

第一作者：徐敏

机构：[1]甘肃中医药大学,兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：26

期号：14

起止页码：53

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(81560746)。

语种：中文

中文关键词：芍药汤;溃疡性结肠炎;Toll样受体4(TLR4);核转录因子-κB p65(NF-κB p65);白细胞介素-6(IL-6)

外文关键词：Shaoyaotang;ulcerative colitis;Toll-like receptor 4(TLR4);nuclear factor-kappaB p65(NF-κB p65);interleukin(6 IL-6)

摘要：目的:以Toll样受体4(TLR4)/核转录因子-κB(NF-κB)信号通路为基础,探讨芍药汤治疗湿热内蕴型溃疡性结肠炎(UC)的作用机制。方法:取50只Wistar大鼠,雌雄各半,采用病证结合方法,以高脂高糖辛辣食物及免疫复合法,联合2,4,6-三硝基苯磺酸(TNBS)结合乙醇复合法复制湿热内蕴型UC大鼠模型。造模成功后,将模型大鼠随机分为模型组、柳氮磺吡啶组及芍药汤低、中、高剂量组,另取10只大鼠(雌雄各半)作为正常组。芍药汤低、中、高剂量组按6,12,24 g·kg^-1灌胃,柳氮磺吡啶组按1 g·kg^-1剂量灌胃,正常组给予等体积生理盐水,连续21 d。末次给药后采集结肠样本,苏木素-伊红(HE)染色观察结肠组织病理变化,运用实时荧光定量聚合酶链式反应(Real-time PCR)检测结肠组织中TLR4,NF-κB p65和IL-6 mRNA的表达,蛋白免疫印迹法(Western blot)检测结肠组织中TLR4,NF-κB p65和IL-6蛋白的表达。结果:与正常组比较,模型组结肠组织损坏较明显,模型组TLR4,NF-κB p65和IL-6 mRNA及蛋白相对表达量明显升高(P<0.05);与模型组比较,柳氮磺吡啶组、芍药汤各剂量组结肠组织损坏明显改善,TLR4,NF-κB p65和IL-6 mRNA及蛋白表达量明显下降(P<0.05),芍药汤各剂量组中以芍药汤高剂量组最为明显。结论:芍药汤可通过调控TLR4/NF-κB通路中TLR4,NF-κB p65和IL-6 mRNA及蛋白的表达,抑制UC病情的发展。
Objective:To explore the mechanism of Shaoyaotang in the treatment of ulcerative colitis(UC)based on toll-like receptor 4(TLR4)/nuclear factor kappaB(NF-κB)signaling pathway.Method:A total of 50 Wistar rats were selected,including half male and half female.The damp-heat UC rat model was replicated by the methods of the combination of diseases and syndromes and the combination of 2,4,6-nitrobenzene sulfonic acid(TNBS)and ethanol.After the successful modeling,the model rats were randomly divided into model group,salazulesulfonate group,and low,medium and high-dose Shaoyaotang groups,and 10 rats(half male and half female)were selected as the blank control group.Low,medium and high-dose Shaoyaotang groups were given 6,12,24 g·kg^-1 by gavage,and salazonyl arsenic group was given 1 g·kg^-1 by gavage.Blank control group was given the equal volume of normal saline for 21 consecutive days.Colon samples were collected after the last administration,and the expressions of TLR4,NF-κB p65 and IL-6 mRNA in colon tissues were detected by fluorescent quantitative polymerase chain reaction(Real-time PCR),and the expressions of TLR4,NF-κB p65 and IL-6 protein in colon tissues were detected by Western blot.Result:Compared with the blank control group,the relative expressions of TLR4,NF-κB p65,IL-6 mRNA and protein in the model group were significantly increased(P<0.05).Compared with the model group,the expression levels of TLR4,NF-κB p65 and IL-6 mRNA and protein in the salazopyridine group and Shaoyaotang groups were significantly decreased(P<0.05).Conclusion:Shaoyaotang can inhibit the development of UC by regulating the expressions of TLR4,NF-κB p65 and IL-6 mRNA and proteins in the TLR4/NF-κB pathway.