文献类型：期刊文献

中文题名：基于化学生物信息学方法探讨黄芪通过调节能量代谢发挥“补气”功效的物质基础和分子机制

英文题名：Exploration on Material Basis and Molecular Mechanism of Astragali Radix Exerting the Effect of “Invigorating Qi” Through Regulating Energy Metabolism Based on Chemo-bio Informatics Methods

作者：后叶虎[1];邱璐[1];靳晓杰[1,2];张敏[2];林佳[2];刘伟[2];魏本君[1];姚娟[1,2];李亚玲[1];刘永琦[1,3]

第一作者：后叶虎

机构：[1]甘肃中医药大学,甘肃省高校重大疾病分子医学与中医药防治研究重点实验室,兰州730000;[2]甘肃中医药大学药学院,兰州730000;[3]甘肃中医药大学敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2023

卷号：40

期号：14

起止页码：1906

中文期刊名：中国现代应用药学

外文期刊名：Chinese Journal of Modern Applied Pharmacy

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：甘肃省基础研究创新群体(20JR10RA332);甘肃省自然科学基金项目(20JR10RA312);2022年度甘肃省高等学校产业支撑计划项目(2022CYZC-54);国家自然科学基金青年基金项目(82104370);甘肃省中医药研究中心开放课题(zyzx-2020-17)。

语种：中文

中文关键词：黄芪;补气;能量代谢;化学生物信息学

外文关键词：Astragali Radix;invigorating Qi;energy metabolism;chemo-bio informatics

摘要：目的 阐释黄芪发挥“补气”功效调节能量代谢的物质基础和生物学机制。方法 TCMSP数据库及文献检索收集黄芪潜在活性成分,SEA数据库进行基于结构相似性的靶点预测,GeneCards、OMIM、TTD数据库获取能量代谢靶点。Cytoscape软件构建黄芪调节能量代谢靶点蛋白-蛋白相互作用网络图,并进行GO和KEGG富集分析。黄芪全成分与关键靶点进行分子对接和层次聚类分析评估靶点-成分亲和力,采用CCK-8法、流式细胞术、ATP试剂盒检测黄芪代表性化合物对H9C2心肌细胞、GES-1胃上皮细胞能量代谢的影响,并进行结合模式分析。结果 网络药理学结果显示,黄芪调节能量代谢潜在作用靶点有126个;GO和KEGG富集分析表明,黄芪调节能量代谢可能与氧化还原过程、蛋白及酶合成的基因表达有关。其中,SIRT1及PPARγ是参与能量代谢调节的关键靶点。分子对接及层次聚类显示黄芪成分对SIRT1及PPARγ具有较好的靶向性,结合分子对接打分值筛选出3个代表性化合物进行体外实验验证;槲皮素、山奈酚能够促进H9C2心肌细胞、GES-1胃上皮细胞能量代谢。结合模式分析表明,槲皮素、山奈酚与SIRT1、PPARγ之间具有较好的结合能力。结论 本研究通过中医药化学生物信息学方法初步阐释了黄芪调节能量代谢的物质基础和生物机制,为黄芪通过“补气”发挥行滞通痹功效的中医内涵提供了科学依据。
OBJECTIVE To explain the material basis and biological mechanism of Astragali Radix's “invigorating Qi”effect to regulate energy metabolism.METHODS The TCMSP database and literature search collected potential active components of Astragali Radix,the SEA database performed target prediction based on structural similarity,and the GeneCards,OMIM,and TTD databases obtained energy metabolism targets.Cytoscape software was used to construct protein-protein interaction network maps of Astragali Radix regulated energy metabolism targets,and GO and KEGG enrichment analyses were performed.Molecular docking and hierarchical cluster analysis were performed to evaluate the target-component affinity between the whole constituents of Astragali Radix and key targets,and the effects of representative compounds of Astragali Radix on the energy metabolism of H9C2 cardiomyocytes and GES-1 gastric epithelial cells were detected,and the binding mode analysis was conducted.RESULTS Network pharmacology results showed that there were 126 potential targets of Astragali Radix regulating energy metabolism.GO and KEGG enrichment analysis showed that Astragali Radix regulating energy metabolism might be related to gene expression of oxidation-reduction process,protein and enzyme synthesis.Among them,SIRT1and PPARγ were key targets involved in the regulation of energy metabolism.Molecular docking and hierarchical clustering showed that Astragali Radix components had superior targeting to SIRT1 and PPARγ,and three representative compounds were selected for in vitro experimental verification in combination with molecular docking scores.Quercetin and kaempferol could promote energy metabolism in H9C2 cardiomyocytes and GES-1 gastric epithelial cells.The binding mode analysis showed that quercetin and kaempferol had preferable binding ability to SIRT1 and PPARγ.CONCLUSION In this study,the material basis and biological mechanism of Astragali Radix regulating energy metabolism are preliminarily explained by traditional Chinese medicine chemo-bio informatics methods,which provide a scientific basis for the connotation of Astragali Radix exerting the effect of stagnation and arthralgia through “invigorating Qi” in traditional Chinese medicine.