文献类型：期刊文献

英文题名：A Novel Caffeoylquinic Acid from Lonicera japonica Exerts Cytotoxic Activity by Blocking the YAP-CTGF Signaling Pathway in Hepatocellular Carcinoma

作者：Shen, Wanying[1,2,3,4];Wei, Xiaofang[1,2,3,4];Li, Yangfang[2,3,5];Liu, Chenxiao[2,3,5];Ge, Lanlan[2,3,5];Yao, Jie[2,3,5];Zeng, Xiaobin[2,3,5];Tang, Xudong[1,4]

第一作者：Shen, Wanying

通信作者：Tang, XD[1];Ge, LL[2];Yao, J[2];Zeng, XB[2];Ge, LL[3];Yao, J[3];Zeng, XB[3];Tang, XD[4];Ge, LL[5];Yao, J[5];Zeng, XB[5]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]Jinan Univ, Shenzhen Peoples Hosp, Ctr Lab Longhua Branch, Dept Infect Dis,Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China;[3]Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China;[4]Res Inst Tsinghua Univ, Key Lab Innovat Tradit Chinese Med & Nat Med, Shenzhen, Guangdong, Peoples R China;[5]Jinan Univ, Ctr Lab Longhua Branch, Dept Infect Dis,Shenzhen Peoples Hosp, 2nd Clin Med Coll, Shenzhen, Guangdong, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Peoples R China;[2]corresponding author), Jinan Univ, Shenzhen Peoples Hosp, Ctr Lab Longhua Branch, Dept Infect Dis,Clin Med Coll 2, Shenzhen, Guangdong, Peoples R China;[3]corresponding author), Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen, Guangdong, Peoples R China;[4]corresponding author), Res Inst Tsinghua Univ, Key Lab Innovat Tradit Chinese Med & Nat Med, Shenzhen, Guangdong, Peoples R China;[5]corresponding author), Jinan Univ, Ctr Lab Longhua Branch, Dept Infect Dis,Shenzhen Peoples Hosp, 2nd Clin Med Coll, Shenzhen, Guangdong, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2023

卷号：33

期号：4

起止页码：736

外文期刊名：REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY

收录：;Scopus(收录号：2-s2.0-85160602554);WOS:【SCI-EXPANDED(收录号:WOS:001000199100001)】；

基金：This work was supported by grants from National Natural Science Foundation of China (81903760, 82204682), Guangdong Basic and Applied Basic Research (2021A1515110841), Shenzhen Science and Technology Program (JCYJ20210324113003007, JCYJ20190806151816859).

语种：英文

外文关键词：New drug; Chinese herbal medicines; Primary liver cancers; In vitro; The Hippo signaling pathway; The Wnt signaling pathway

摘要：We have purified a novel caffeoylquinic acid named 3,4-di-O-caffeoylquinic acid isobutyl ester from the flower buds of Lonicera japonica Thunb., Caprifoliaceae. However, the biological function of 3,4-di-O-caffeoylquinic acid isobutyl ester is still unknown. Here, we found that 3,4-di-O-caffeoylquinic acid isobutyl ester effectively inhibited the proliferation and migration of hepatocellular carcinoma cells, and it displayed less toxicity to a normal liver cell line. Mechanistic studies showed that 3,4-di-O-caffeoylquinic acid isobutyl ester diminished the expression of YAP at the mRNA level. Overexpression of YAP significantly rescued HepG2 cells from the 3,4-di-O-caffeoylquinic acid isobutyl ester-induced suppression of proliferation and migration. Furthermore, the YAP downstream target gene CTGF was significantly repressed upon 3,4-di-O-caffeoylquinic acid isobutyl ester treatment, which was ameliorated by YAP overexpression. In addition, 3,4-di-O-caffeoylquinic acid isobutyl ester decreased the expression of ss-catenin as well as CDK4/6. Collectively, 3,4-di-O-caffeoylquinic acid isobutyl ester exerts antihepatocellular carcinoma activity by inhibiting the YAP-CTGF pathway which controls the proliferation and migration of hepatocellular carcinoma cells. The Wnt/ss-catenin pathway might be another pathway by which 3,4-di-O-caffeoylquinic acid isobutyl ester exerts antihepatocellular carcinoma activity. As a novel natural compound, 3,4-di-O-caffeoylquinic acid isobutyl ester might be a promising agent for hepatocellular carcinoma therapy.