文献类型：期刊文献

中文题名：基于网络药理学及实验验证探讨香砂六君子汤治疗功能性消化不良作用机制

英文题名：Exploration of Mechanism of Xiangsha Liujunzi Decoction in the Treatment of Functional Dyspepsia Based on Network Pharmacology and Experimental Verification

作者：赵琳娜[1,2];成映霞[3];白敏[1,2];刘梦雅[1,2];李润法[1,2];高晗[4];安耀荣[1];段永强[3]

第一作者：赵琳娜

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃省实验动物行业技术中心,甘肃兰州730000;[3]宁夏医科大学中医学院,宁夏银川750004;[4]石家庄傅山肿瘤医院,河北石家庄050000

第一机构：甘肃中医药大学

年份：2023

卷号：30

期号：3

起止页码：7

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2023_2024】；

基金：宁夏回族自治区重点研发计划项目-引才专项(2022BSB03080);甘肃中医药大学研究生创新创业基金(2022CX06)。

语种：中文

中文关键词：网络药理学;功能性消化不良;香砂六君子汤;丝裂原活化蛋白激酶

外文关键词：network pharmacology;functional dyspepsia;Xiangsha Liujunzi Decoction;MAPK

摘要：目的运用网络药理学预测分析香砂六君子汤干预功能性消化不良(FD)的作用机制,并通过动物实验进行验证。方法通过TCMSP获取香砂六君子汤主要成分及对应靶点,运用GeneCards数据库获取FD疾病靶点;筛选出药物与疾病共同靶点并利用Cytoscape3.7.1软件进行药物-成分-靶点-疾病网络构建;采用STRING数据库构建靶点蛋白相互作用(PPI)网络,通过拓扑分析筛选核心靶点;采用Metascape进行GO功能及KEGG通路富集分析。建立脾胃虚弱型FD大鼠模型,观察香砂六君子汤干预效果,检测核心靶点在十二指肠组织中的表达。结果筛选得到香砂六君子汤与FD共同靶点151个,PPI网络分析发现,其关键靶点为MAPK1、MAPK14、JUN、IL-6等,KEGG通路富集分析得到203条通路,多与炎症相关。动物实验结果显示,与空白组比较,模型组大鼠体质量增长缓慢、进食量减少,胃组织病理结构无明显异常但十二指肠绒毛排列散乱、有炎性细胞浸润,十二指肠组织IL-6、TNF-α、IL-1β含量显著升高(P<0.01),p-ERK、p-P38MAPK、p-NF-κBp65蛋白表达显著升高(P<0.01);与模型组比较,各给药组大鼠体质量及进食量明显增加,十二指肠绒毛排列紧密、炎性浸润减少,IL-6、TNF-α、IL-1β含量及p-ERK、p-P38MAPK、p-NF-κBp65蛋白表达均有不同程度下降。结论香砂六君子汤能通过下调p-ERK、p-P38MAPK及下游p-NF-κBp65水平调控十二指肠低度炎症,改善FD症状。
Objective To predict and analyze the mechanism of Xiangsha Liujunzi Decoction in the intervention of functional dyspepsia(FD)by network pharmacology;To further verify it by animal experiments.Methods The main components and corresponding targets of Xiangsha Liujunzi Decoction were obtained by TCMSP,and the disease targets of FD were obtained by GeneCards database;the common targets of medicine and disease were screened and Cytoscape 3.7.1 software was used to construct the medicine-component-target-disease network;STRING online database was used to construct PPI network of target protein and topological analysis was used to screen core targets;GO function and KEGG pathway enrichment analysis of gene ontology were carried out on metascape website;the FD model rats with spleen stomach deficiency was reconstructed,and the intervention effect of Xiangsha Liujunzi Decoction was observed.The core target expression in duodenal tissue was detected.Results The results of network pharmacology showed that 151 common targets of Xiangsha Liujunzi Decoction and FD were obtained through screening.PPI network analysis found that the key targets were MAPK1,MAPK14,JUN and IL-6.KEGG pathway enrichment analysis results obtained a total of 203 related pathways,mostly related to inflammation.The experimental results showed that compared with the blank group,the body mass of the model group increased slowly,the amount of food intake decreased,and there was no obvious abnormality in the pathological structure of gastric tissue,but there were scattered villi and inflammatory cell infiltration in duodenal tissue,the contents of IL-6,TNF-α and IL-1β increased significantly(P<0.01),and the expressions of p-ERK,p-P38MAPK,p-NF-κBp65 increased significantly(P<0.01);compared with the model group,the body mass and food intake of rats in each administration group increased significantly,the duodenal villi were closely arranged,the inflammatory infiltration was reduced,and the contents of IL-6,TNF-α,IL-1β and expressions of p-ERK,p-P38MAPK,and p-NF-κBp65decreased in varying degrees.Conclusion Xiangsha Liujunzi Decoction can down-regulate levels of p-ERK,p-P38MAPK,and downstream p-NF-κBp65 to regulate low-grade duodenal inflammation and improve FD symptoms.