文献类型：期刊文献

中文题名：生长年限对甘肃贝母根际土壤细菌群落结构的影响

英文题名：Effects of growth years on soil bacterial community structure in rhizosphore soil of Fritillaria przewalskii

作者：武睿[1,3];陈垣[1];郭凤霞[2];周洋[1];焦旭升[1]

第一作者：武睿

机构：[1]甘肃农业大学农学院,甘肃省中药材规范化生产技术创新重点实验室,甘肃省药用植物栽培育种工程研究中心,甘肃兰州730070;[2]甘肃农业大学生命科学技术学院,甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室,甘肃兰州730070;[3]甘肃中医药大学定西校区,甘肃定西743000

第一机构：甘肃农业大学农学院,甘肃省中药材规范化生产技术创新重点实验室,甘肃省药用植物栽培育种工程研究中心,甘肃兰州730070

年份：2021

卷号：39

期号：6

起止页码：153

中文期刊名：干旱地区农业研究

外文期刊名：Agricultural Research in the Arid Areas

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2021_2022】；

基金：国家自然科学基金项目(31660158,31360316);甘肃省中药材产业科技攻关项目(GYC12-06)。

语种：中文

中文关键词：甘肃贝母;高通量测序;细菌群落;多样性

外文关键词：Fritillaria przewalskii;high-throughput sequencing;bacterial community;diversity

摘要：采用Illumina MiSeq高通量测序技术对撂荒地(CK)、生长1 a(BM-1Y)、3 a(BM-3Y)和5 a(BM-5Y)的甘肃贝母根际土壤细菌16S rRNA基因V3-V4可变区进行测序分析,探究不同生长年限甘肃贝母对根际土壤细菌群落结构的影响。结果表明:生长年限对甘肃贝母土壤理化因子和细菌菌群均有影响,其中BM-5Y处理显著降低了土壤有机质(OM)、水解氮(HN)和速效磷(AP)的含量(P<0.05),较CK处理分别降低了22.76%、9.28%、51.25%;BM-5Y处理显著降低根际土壤细菌多样性(P<0.05),其中OUT数、PD值及Chao I指数较CK处理分别降低了7.23%、6.79%、6.47%。生长年限改变了细菌群落在门和属水平的群落组成,但各生长年限中的优势菌门(相对丰度>5%)相对稳定,均为放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi),其在CK、BM-1Y、BM-3Y和BM-5Y处理中的相对丰度总和分别为82.28%、84.16%、83.12%和82.30%。土壤pH、OM、HN、AP及AK含量是驱动根际土细菌群落的主要环境因子,其程度大小为:pH>AK>HN>AP>OM。土壤理化因子与各优势细菌门的相关性不同,pH与放线菌门(Actinobacteria)和芽单胞菌门(Gemmatimonadetes)呈显著负相关(P<0.05),OM、HN和AP均与厚壁菌门(Firmicutes)呈显著负相关(P<0.05)。综上,甘肃贝母生长年限延长(BM-5Y)会显著降低土壤的养分含量和细菌群落的多样性。
In this study,the V3-V4 aera of 16S rRNA gene of bacteria from the Fritillaria przewalskii rhizosphere soil with cultivated land abandonment(CK),1 year(BM-1Y),3 years(BM-3Y)and 5 years(BM-5Y)were sequenced by high-throughput sequencing,and the effect of different growth years of Fritilaria przewalskii on the bacterial community structure of rhizosphere soil was discussed.The results showed that the growth years of Fritillaria przewalskii had an impact on both physical and chemical properties and bacterial consortium of soil.BM-5Y treatment significantly reduced organic matter(OM),hydrolyzed nitrogen(HN)and available phosphorus(AP)content(P<0.05)by 22.76%,9.28%,and 51.25%respectively when compared with CK.BM-5Y significantly reduced the bacterial diversity of the rhizosphere soil(P<0.05).Of which,the OUT number,PD value and Chao I index were reduced by 7.23%,6.79%,and 6.47%respectively compared with CK.Growth years changed the composition of bacterial communities at the phylum and genus level.However,the dominant bacteria phyla(relative abundance>5%)were consistent in all treatments,and they were Actinobacteria,Proteobacteria,Acidobacteria and Chloroflexi.The sum of relative abundances in CK,BM-1Y,BM-3Y and BM-5Y were 82.28%,84.16%,83.12%and 82.30%,respectively.Soil pH,organic matter,hydrolyzed nitrogen,available phosphorus,and available potassium content were the important driving factors the bacterial community in the rhizosphere,and their degree was:pH>AK>HN>AP>OM.The physicochemical properties of soil had different correlations with the dominant bacteria phyla.pH was significantly negatively correlated with Actinobacteria and Gemmatimonadetes(P<0.05).Organic matter,hydrolyzed nitrogen and available phosphorus were significantly negatively correlated withFirmicutes(P<0.05).In summary,the nutrient content and diversity of the bacterial communities of the soil were significantly reduced with the extension of growth years(BM-5Y)in Fritillaria przewalskii.