文献类型：期刊文献

中文题名：紫花高乌头药材的TLC鉴别及主要生物碱的测定

英文题名：TLC identification and the content determination of the alkaloids in Aconitum excelsum

作者：史巧霞[1];朱应怀[1,2];靳子明[1]

第一作者：史巧霞

机构：[1]甘肃中医药大学附属医院制剂中心,甘肃兰州730000;[2]甘肃中医药大学甘肃省中藏药化学与质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2019

卷号：34

期号：4

起止页码：375

中文期刊名：华西药学杂志

外文期刊名：West China Journal of Pharmaceutical Sciences

收录：CSTPCD;;CSCD:【CSCD_E2019_2020】；

基金：国家自然科学基金资助项目(批准号:81460611);甘肃省中医药管理局项目(编号:GZK-2013-54);甘肃中医药大学附属医院院内创新基金项目(编号:gzfy-2018-05)

语种：中文

中文关键词：紫花高乌头;质量控制;定性检查;薄层色谱;高效液相色谱;高乌甲素;冉乌头碱

外文关键词：Aconitum excelsum Reichb;Quality control;Qualitative identification;TLC;HPLC;Lappacontine;Ranaconitine

摘要：目的对紫花高乌头药材进行TLC检查并测定其主要生物碱高乌甲素、冉乌头碱的含量。方法采用TLC法对紫花高乌头进行定性鉴别;采用HPLC法测定药材中乌甲素、冉乌头碱的含量;按《中国药典》中的方法测定其水分、灰分与浸出物的含量。结果紫花高乌头药材的TLC斑点清晰,分离度好;暂定药材的水分、总灰分及酸不溶性灰分分别不得过10.0%、11.0%、4.5%,醇溶性浸出物应不少于24.0%;紫花高乌头药材中高乌甲素、冉乌头碱的含量应不少于0.126%、0.018%。结论所用方法简便准确,可用于紫花高乌头药材的质量评价。
OBJECTIVE To identify Aconitum excelsum. Reichb by TLC and to determine the conlenls of lappacontine and ranaconitine in A. excelsum. METHODS The TLC method was used for the qualitative identification of A. excelsum. The contents of lappacontine and ranaconitine were determined by HPLC. At the same time, the moisture, ash and extractives were detected by the methods published in pharmacopeia. RESULTS The TLC results showed the spots developed clearly and the separation was good. The moisture, total ash and acid insoluble ash of A. excelsum were respectively less than 10. 0%,11.0% and 4. 5%,and the alcohol soluble extractives were not less than 24. 0%. The lappacontine and ranaconitine of A. excelsum were not less than 0. 126% and 0. 018%,respectively. CONCLUSION The method was sample and accurate, and it can be used for the quality control of A. excelsum.