文献类型：期刊文献

英文题名：The role of radix hedysari polysaccharide on the human umbilical vein endothelial cells (HUVECs) induced by high glucose

作者：Liu, Jing[1];Deng, Wenjuan[2];Fan, Lei[2];Tian, Limin[1];Jin, Liying[3];Jin, Zhisheng[4];Guo, Qian[1];Xu, Yanjia[1];Li, Ning[3]

第一作者：Liu, Jing

通信作者：Liu, J[1]

机构：[1]Peoples Hosp Gansu Prov, Dept Endocrinol, Lanzhou 730000, Gansu, Peoples R China;[2]Hebei No Inst, Attached Hosp 1, Zhangjiakou 075000, Hebei, Peoples R China;[3]Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Gansu, Peoples R China;[4]Gansu Coll Tradit Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Peoples Hosp Gansu Prov, Dept Endocrinol, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Peoples Hosp Gansu Prov, Dept Endocrinol, 204 Donggang W Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2012

卷号：23

期号：3

起止页码：287

外文期刊名：EUROPEAN JOURNAL OF INTERNAL MEDICINE

收录：;Scopus(收录号：2-s2.0-84858752415);WOS:【SCI-EXPANDED(收录号:WOS:000301125900024)】；

基金：This study was supported by the Department of Endocrinology, People's Hospital of Gansu Province, Lanzhou, China and the Medical Experiment Center of Lanzhou University, Lanzhou, China. We are grateful to Mr Zhi-Sheng Jin for contributing to HPS. All the authors have read and approved the submission of this paper. Contract grant sponsor: Technology Project of Traditional Chinese Medicine of Gansu Province in China; contract grant number: YZK2009-13.

语种：英文

外文关键词：Radix hedysari polysaccharide; Endothelial cells; Apoptosis; JNK; Caspase

摘要：Background: Diabetes mellitus can cause a wide variety of vascular complications and it is one of the major risk factors for cardiovascular diseases (CVD). High glucose can induce vascular endothelial cell apoptosis. In this study, we investigated the effect of radix hedysari polysaccharide (HPS) on the depression of apoptosis of human umbilical vein endothelial cells (HUVECs) induced by high glucose. Methods: HUVECs were treated with media containing 30 mM glucose in the presence or absence of vitamin C or HPS. The level of intracellular reactive oxygen species (ROS) and apoptosis of HUVECs was measured with flow cytometry. Expression of c-Jun NH2-terminal kinase (JNK) and caspase-3 were testified by real-time quantitative RT-PCR and immunofluorescence. Results: High glucose was capable of eliciting the overexpression of JNK during the treatment procedure. Moreover, we found that the caspase-3 became overexpressed in apoptosis induced by high glucose; HPS could inhibit apoptosis under high glucose and suppress the generation of ROS and the overexpression of JNK and caspase-3. The effect of HPS on ROS quenching, inhibition of JNK and caspase-3 overexpression at the concentration of 100 mu g/ml was similar to that of vitamin C at the concentration of 100 mu M. Conclusion: The findings of the present study may suggest that HPS play a protection role on HUVECs against apoptosis induced by high glucose. (C) 2011 European Federation of Internal Medicine. Published by Elsevier B.V. All rights reserved.