文献类型：期刊文献

中文题名：脱管散对大鼠压力性损伤创面愈合及创面组织中TNF-α、IL-6表达的影响

英文题名：Effects of Tuoguan San on Wound Healing and Expression of TNF-α and IL-6 in Wound Tissue After Pressure Injury in Rats

作者：战青[1];张乐乐[1];王波[1];宋旭林[1];车莹[1]

第一作者：战青

机构：[1]甘肃中医药大学护理学院,甘肃兰州730000

第一机构：甘肃中医药大学护理学院

年份：2023

卷号：19

期号：5

起止页码：22

中文期刊名：亚太传统医药

外文期刊名：Asia-Pacific Traditional Medicine

基金：甘肃省中医药管理局项目(GZKP-2020-37);甘肃省中医方药挖掘与创新转化重点实验室开放课题(zyzx-2020-13)。

语种：中文

中文关键词：压力性损伤;创面;愈合率;TNF-α;IL-6;脱管散

外文关键词：Pressure Injury;The Wound;The Healing Rate;TNF-α;IL-6;Tuoguan San

摘要：目的:观察脱管散对大鼠压力性损伤创面愈合及创面组织TNF-α、IL-6表达的影响。方法:将180只SPF级Wistar大鼠随机分出30只大鼠为空白对照组,其余150只大鼠建造I/R损伤大鼠压力性损伤模型,判断造模成功后随机分为模型组、湿润烧伤膏组及脱管散低、中、高剂量组,每组各30只。分别于连续用药后第3天、7天、14天计算创面愈合率,采用免疫荧光技术检测创面组织中TNF-α和IL-6的表达水平。结果:湿润烧伤膏组和脱管散各组的创面愈合率均高于模型组(P<0.05),除用药后第3天,脱管散中剂量组无显著优势(P>0.05)外,在用药后第3天、7天、14天,脱管散低剂量组与湿润烧伤膏组均可有效促进创面愈合(P>0.05);脱管散低剂量组优于中、高剂量组(P<0.05)。创面组织TNF-α、IL-6表达:除空白组外,各组TNF-α、IL-6表达在用药后第3天、7天、14天均呈先升高、后下降的“单峰”趋势,高峰出现在用药后第7天。模型组3个时间节点均高于其他各组(P<0.05);湿润烧伤膏组3个时间节点均低于脱管散各组(P<0.05),与脱管散低剂量组效果相当(P>0.05);脱管散各组3个时间节点低剂量组均优于中、高剂量组(P<0.05)。结论:在不同时间点,低剂量脱管散与湿润烧伤膏均具有促进创面愈合,缩小创面面积,提高创面愈合率的优势;伴随创面愈合进展,脱管散对TNF-α与IL-6的表达具有双向调节作用,即创面修复早期上调TNF-α与IL-6表达以启动免疫反应、吞噬微生物及坏死组织为创面愈合营造良好微环境,创面愈合后期下调TNF-α与IL-6表达以促进创面由炎症期向增殖期过渡,对促进压力性损伤创面愈合具有一定优势。
Objective:To observe the effect of Tuoguan San on wound healing and the expression of TNF-α and IL-6 in wound tissue of rats with pressure injury.Methods:80 SPF Wistar rats were randomly divided into 30 rats as the blank control group,and the other 150 rats were constructed into the rat pressure injury model of I/R injury.After modeling,the rats were randomly divided into model group,MEBO group,and Tuoguan San low-dose,medium-dose and high-dose groups,with 30 rats in each group.The wound healing rate was calculated on the 3d,7d,and 14d after continuous medication,and the expression levels of inflammatory factors TNF-α and IL-6 in wound tissues were detected by immunofluorescence(IF).Results:Wound healing rate:the MEBO group and Tuoguan San groups were higher than that of the model group(P0.05),the low-dose Tuoguan San group and the MEBO group could effectively promote wound healing on days 3,7 and 14 after medication(P>0.05).The low dose Tuo guan san group was better than the medium and high dose groups(P<0.05).Influence of TNF-α and IL-6 expression in wound tissue:Except for the blank group,the expressions of TNF-α and IL-6 in each group showed a"single peak"trend of increasing first and then decreasing at 3,7 and 14 days after medication,and the peak occurred on the 7th day after medication.The three time nodes in the model group were higher than those in the other groups(P<0.05).The three time points in the MEBO group were lower than those in the Tuoguan San group(P<0.05),and the effect was similar to that in the Tuoguan San low-dose group(P>0.05).The low-dose group was better than the medium-dose and high-dose groups in the three time nodes of Tuoguan San(P