文献类型：期刊文献

中文题名：大鼠心肌缺血再灌注损伤模型的改进与评判

英文题名：Improvement and evaluation of the rat model of myocardial ischemia reperfusion injury

作者：张卫强[1];王涛[1];张志明[2];雍文兴[2];刘永琦[1];李娟[2];梁艳[1]

第一作者：张卫强

机构：[1]甘肃中医药大学,兰州730000;[2]甘肃中医药大学附属医院,兰州730000

第一机构：甘肃中医药大学

年份：2018

卷号：26

期号：3

起止页码：311

中文期刊名：中国实验动物学报

外文期刊名：Acta Laboratorium Animalis Scientia Sinica

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金项目资助(No.81660730)~~

语种：中文

中文关键词：大鼠;心肌缺血再灌注损伤;动物模型

外文关键词：rats;myocardial ischemia-reperfusion injury;animal models

摘要：目的改进大鼠急性心肌缺血再灌注损伤(MI/RI)模型的制备方法,并进行综合评判,为后续开展MI/RI研究奠定良好的模型基础。方法将36只清洁级Wistar大鼠随机分为3组:正常组(Normal组),不干预左冠状动脉前降支(LAD);假手术组(Sham组),仅穿线,不结扎LAD;缺血再灌注组(MI/RI组),结扎LAD30 min,再灌注120 min。大鼠于麻醉后连续记录心电图,气管切开行呼吸机辅助通气,开胸对大鼠LAD分别进行干预后从心电图、心肌酶含量测定、心梗面积及心肌病理组织学检查等方面对改进后的模型进行综合评判。结果 MI/RI组心电图有明显ST-T动态变化;MI/RI组肌酸激酶同工酶(CK-MB)水平于2 h后显著升高;与Sham组比较,MI/RI组梗死面积明显增大;MI/RI组心肌细胞变性坏死明显,炎性细胞广泛浸润。结论改进后的造模方法不但降低了手术难度,而且成功建立了大鼠MI/RI模型,为后续开展MI/RI研究奠定了良好的模型基础。
Objective To improve the preparation method of rat model of acute myocardial ischemia reperfusion injury（ MI/RI）,to make a comprehensive evaluation and to lay a good model foundation for following MI/RI research.Methods Thirty-six clean grade Wistar rats were randomly divided into 3 groups： normal group without intervention of the left anterior descending coronary artery（ LAD）,sham operation group with only wearing thread but no ligation of the coronary artery,and ischemia reperfusion group,with ligation of the LAD for 30 min and reperfusion for 120 min. Rats after anesthesia had continuous ECG recording,and tracheostomy for mechanical auxiliary ventilation. Thoracotomy and LAD intervention were carried out on the rats,and then the rat models were comprehensively evaluated by electrocardiography,detection of myocardial enzyme content,determination of the infarct size,and pathological examination of the myocardium. Results The electrocardiogram of the MI/RI group showed obvious ST-T dynamic changes. The level of CK-MB in the MI/RI group was significantly increased at 2 h after surgery. Compared with the sham group,the infarct size of the MI/RI group was significantly increased,with evident degeneration and necrosis of cardiomyocytes and extensive inflammatory cell infiltration in the myocardium. Conclusions The improved modeling method not only reduced the difficulties of operation,but has also successfully established the rat model of MI/RI,providing a good foundation for further MI/RI research.