文献类型：期刊文献

中文题名：基于16S rRNA序列分析糖皮质激素诱导大鼠骨质疏松模型肠道菌群的变化

英文题名：The intestinal microflora changes in rat osteoporosis model induced by glucocorticoid based on 16S rDNA sequence analysis

作者：董万涛[1];宋敏[2];巩彦龙[2];董平[2];田杰祥[1];张亚彬[2];文浩楠[2]

第一作者：董万涛

机构：[1]甘肃中医药大学附属医院,甘肃兰州730020;[2]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学第二附属医院

年份：2020

卷号：26

期号：9

起止页码：1291

中文期刊名：中国骨质疏松杂志

外文期刊名：Chinese Journal of Osteoporosis

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：国家自然科学基金项目(81960878);甘肃省创新基地和人才计划-自然科学基金计划项目(17JR5RA056,1506RJZA048);甘肃省中医药防治慢性疾病重点实验室开放基金项目(GSMBKY2015-07)。

语种：中文

中文关键词：糖皮质激素;骨质疏松;肠道菌群;结构及功能;16S rRNA

外文关键词：glucocorticoid;osteoporosis;intestinal flora;structure and function;16S rRNA

摘要：目的通过16S rRNA序列分析探讨糖皮质激素诱导大鼠骨质疏松模型肠道菌群结构及功能的变化。方法将20只Wistar大鼠,随机分为模型组(K组,10只)正常组(MY组,10只),模型组采用糖皮质激素诱导法复制骨质疏松模型,正常组正常饲养,8周后每组随机选取4只收集大鼠粪便,提取并检测粪菌总DNA,检测样品质量,肠道菌群OTU分析、香农指数曲线分析、NMDS分析,16S rRNA扩增子测序,对标本中的细菌16S rRNA V3-V4进行定性分析、16S rRNA功能基因预测分析。结果经样品质量控制及甲醛变性琼脂糖凝胶电泳检测提示样品质量合格;K组与MY组共同含有575个OTU数据,K组和MY组分别有12个不同的OUT数据;当香农指数为3~4时,每个样本抽取的测序条数发现的物种趋于饱和;NMDS分析显示stress=0.05<0.2;与正常组比较,模型照组大鼠肠道菌群的多样性增加;功能主要以细胞运动、能量生产和转换、防御机制、转录、碳水化合物转运和代谢为主。结论糖皮质激素诱导大鼠骨质疏松模型肠道菌群在多样性、结构及功能方面发生了较大变化,肠道微生态细胞运动、能量生产和转换、防御、转录、碳水化合物转运和代谢等功能失衡可能为糖皮质激素诱发骨质疏松症的发病机制之一。
Objective To investigate the changes in the structure and function of intestinal flora in glucocorticoid inducted osteoporosis model in rats with 16S rRNA sequence analysis.Methods Twenty Wistar rats were randomly divided into the model group(group K,n=10)and normal group(group MY,n=10).Rats in model group were induced to osteoporosis with glucocorticoid.Rats in normal group were raised normally.After 8 weeks,4 rats in each group was randomly selected and their feces was collected.The total DNA and sample quality of the feces,the OTU analysis of the intestinal flora,the Shannon diversity index curve analysis,the NMDS analysis,and the 16S rRNA amplicon sequencing were performed.Qualitative analysis of bacteria in the specimen and predictive analysis of 16SrRNA functional genes were conducted.Results Sample quality control and formaldehyde denaturing agarose gel electrophoresis showed that the sample quality was qualified.K group and MY group contained 575 common OUT data.There were 12 different OTU data in group K and group MY.When the Shannon diversity index was 3-4,the number of sequencing strips extracted from each sample tended to be saturated.NMDS analysis showed that the stress was 0.05 and less than 0.2.Compared with that in the normal group,the diversity of the intestinal flora in the model group increased.The function was mainly based on cell motion,energy production and conversion,defense mechanisms,transcription,carbohydrate transport,and metabolism.Conclusion The intestinal microflora in glucocorticoids-induced osteoporosis rats have changed greatly in diversity,structure,and function.The imbalance of intestinal microecological cell movement,energy production and conversion,defense,transcription,carbohydrate transport,and metabolism may be one of the pathogenesis of osteoporosis induced by glucocorticoids.